| Author | Iñiguez, Alena M. | |
| Author | Reinhard, Karl J. | |
| Author | Ferreira, Luiz Fernando | |
| Author | Vicente, Ana Carolina P. | |
| Access date | 2020-11-14T19:25:19Z | |
| Available date | 2020-11-14T19:25:19Z | |
| Document date | 2003 | |
| Citation | IÑIGUEZ, Alena Mayo et al. Enterobius vermicularis: ancient DNA from north and south American human coprolites. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, Suppl. 1, p. 67-69, 2003. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44421 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | DNA Antigo | pt_BR |
| Subject in Portuguese | Enterobius vermicularis | pt_BR |
| Subject in Portuguese | Coprólitos | pt_BR |
| Title | Enterobius vermicularis: Ancient DNA from North and South American Human Coprolites | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762003000900013 | |
| Abstract | A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Nebraska-Lincoln. School of Natural Resource Sciences. Lincoln, NE, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Ancient DNA | pt_BR |
| Subject | Enterobius vermicularis | pt_BR |
| Subject | Coprolites | pt_BR |
| e-ISSN | 1678-8060 | |
