| Author | Pinto, Patrícia M. | |
| Author | Resende, Maria A. | |
| Author | Koga-Ito, Cristiane Y. | |
| Author | Tendler, Miriam | |
| Access date | 2020-11-18T20:27:02Z | |
| Available date | 2020-11-18T20:27:02Z | |
| Document date | 2004 | |
| Citation | PINTO, Patrícia M. et al. Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 2, p. 147-152, Mar. 2004. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44525 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Candida spp. | pt_BR |
| Subject in Portuguese | Reação em cadeia de polimerase | pt_BR |
| Subject in Portuguese | Candidose | pt_BR |
| Subject in Portuguese | Técnica de Amplificação ao Acaso de DNA Polimórfico | pt_BR |
| Title | Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762004000200006 | |
| Abstract | The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from
different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis
using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are
efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in
providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid
identification of pathogenic fungi. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | UNESP. aculdade de Odontologia de São José dos Campos. Departamento de Biociências e Diagnóstico Bucal. São José dos Campos. SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Polymerase chain reaction | pt_BR |
| Subject | Random amplified polymorphic DNA | pt_BR |
| Subject | Candidosis | pt_BR |
| Subject | Candida spp. | pt_BR |
| DeCS | Técnica de Amplificação ao Acaso de DNA Polimórfico | pt_BR |
| e-ISSN | 1678-8060 | |
