| Author | Cunha, J. M. Albuquerque | |
| Author | Mello, C. B. | |
| Author | Garcia, Eloi S. | |
| Author | Azambuja, P. | |
| Author | Souza, W. de | |
| Author | Gonzalez, M. S. | |
| Author | Nogueira, N. F. S. | |
| Access date | 2020-11-19T17:59:22Z | |
| Available date | 2020-11-19T17:59:22Z | |
| Document date | 2004 | |
| Citation | CUNHA, J. M. Albuquerque et al. Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 9, p. 815-822, Dec. 2004. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44544 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Microvilosidades | pt_BR |
| Subject in Portuguese | Triatomíneos | pt_BR |
| Subject in Portuguese | Membranas perimicrovilares | pt_BR |
| Subject in Portuguese | Hormônios | pt_BR |
| Title | Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762004000800006 | |
| Abstract | The effects of blood components, nerve-cord severance, and ecdysone therapy on the posterior midgut epithelial
cells of 5th-instar Rhodnius prolixus nymphs 10 days after feeding were analyzed by transmission electron microscopy. Cutting the nerve-cord of the blood-fed insects partially reduced the development of microvilli and
perimicrovillar membranes (PMM), and produced large vacuoles and small electrondense granules; insects fed on
Ringer’s saline diet exhibited well developed microvilli and low PMM production; swolled rough endoplasmatic
reticulum and electrondense granules; Ringer’s saline meal with ecdysone led to PMM development, glycogen
particles, and several mitochondria in the cytoplasm; epithelial cells of the insects fed on Ringer’s saline meal whose
nerve-cord was severed showed heterogeneously distributed microvilli with reduced PMM production and a great
quantity of mitochondria and glycogen in the cytoplasm; well developed microvilli and PMM were observed in
nerve-cord severed insects fed on Ringer’s saline meal with ecdysone; Ringer’s saline diet containing hemoglobin
recovered the release of PMM; and insects fed on human plasma showed slightly reduced PMM production,
although the addition of ecdysone in the plasma led to a normal midgut ultrastructural organization. We suggest
that the full development of microvilli and PMM in the epithelial cells depends on the abdominal distension in
addition to ingestion of hemoglobin, and the release of ecdysone. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultra-estrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
| Subject | Triatomine | pt_BR |
| Subject | Midgut | pt_BR |
| Subject | Perimicrovillar membranes | pt_BR |
| Subject | Hormone | pt_BR |
| Subject | Microvilli | pt_BR |
| e-ISSN | 1678-8060 | |
