| Author | Albuquerque, Pedro Pereira Lira Furtado de | |
| Author | Santos, Luciana H. S. | |
| Author | Antunes, Deborah | |
| Author | Caffarena, Ernesto Raul | |
| Author | Fifueiredo, Andreza Soriano | |
| Access date | 2020-12-07T19:52:04Z | |
| Available date | 2020-12-07T19:52:04Z | |
| Document date | 2020 | |
| Citation | ALBUQUERQUE, Pedro Pereira Lira Furtado de et al. Structural insights into NS5B protein of novel equine hepaciviruses and pegiviruses complexed with polymerase inhibitors, Virus Research, v. 278, 197867, p. 1-9, Jan. 2020. | pt_BR |
| ISSN | 0168-1702 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44730 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hepacivírus | pt_BR |
| Subject in Portuguese | Acoplamento molecular | pt_BR |
| Subject in Portuguese | Pegivírus | pt_BR |
| Subject in Portuguese | Inibidor de polimerase | pt_BR |
| Subject in Portuguese | Modelagem comparativa | pt_BR |
| Subject in Portuguese | NS5B | pt_BR |
| Title | Structural insights into NS5B protein of novel equine hepaciviruses and pegiviruses complexed with polymerase inhibitors | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.virusres.2020.197867 | pt_BR |
| Abstract | Infections produced by hepaciviruses have been associated with liver disease in horses. Currently, at least three viruses belonging to the Flaviviridae family are capable of producing a chronic infection in equines: non-primate hepacivirus (NPHV), Theiler’s disease-associated virus (TDAV), and equine pegivirus (EPgV). The RNA-dependent RNA polymerases of viruses (RdRp) (NS5 protein), from the flavivirus family, use de novo RNA synthesis to initiate synthesis. The two antiviral drugs currently used to treat hepatitis C (HCV), sofosbuvir and dasabuvir, act on the viral NS5B polymerase as nucleoside and non-nucleoside inhibitors, respectively. Both drugs have shown significant clinical inhibition of viral response. In this work, we aimed to model the NS5B polymerase of the equine hepacivirus (EHCV) subtypes 1 and 2, TDAV and EPgV, to assess whether current direct-acting antiviral drugs against HCV interact with these proteins. Crystal structures of HCV-NS5B were used as templates for modeling target sequences in both conformations (open and closed). Also, molecular docking of sofosbuvir and dasabuvir were performed to predict their possible binding modes at the modeled NS5B polymerase binding sites. We observed that the NS5B models of the EHCV and EPgV shared well-conserved 3D structures to HCV-NS5B and other RdRps, suggesting functional conservation. Interactions of EHCV subtypes 1, 2 and TDAV polymerases with sofosbuvir showed a similar molecular interaction pattern compared to HCV-NS5B, while interactions with dasabuvir were less conserved. In silico studies of molecular interactions between these modeled structures and sofosbuvir suggest that this compound could be efficient in combating equine pathogens, thus contributing to animal welfare. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Modelagem Molecular e Planejamento de Fármacos.. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Grupo de Biofísica Computacional e Modelagem Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hepacivirus | pt_BR |
| Subject | Pegivirus | pt_BR |
| Subject | NS5B | pt_BR |
| Subject | Polymerase inhibitor | pt_BR |
| Subject | Comparative modeling | pt_BR |
| Subject | Molecular docking | pt_BR |
