| Author | Roges, Emily Moraes | |
| Author | Gonçalves, Verônica Dias | |
| Author | Cardoso, Maira Duarte | |
| Author | Festivo, Marcia Lima | |
| Author | Siciliano, Salvatore | |
| Author | Berto, Lucia Helena | |
| Author | Pereira, Virginia Leo de Almeida | |
| Author | Rodrigues, Dalia dos Prazeres | |
| Author | Aquino, Maria Helena Cosendey de | |
| Access date | 2020-12-14T20:09:46Z | |
| Available date | 2020-12-14T20:09:46Z | |
| Document date | 2020 | |
| Citation | ROGES, Emily Moraes et al. Virulence Associated Genes and Antimicrobail Resistance of Aeromonas hydrophila isolates from Animal, Food and Human sources in Brazil. BioMed Research International, v. 2020, 1052607, p. 1-8, May 2020. | pt_BR |
| ISSN | 2314-6133 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44876 | |
| Language | por | pt_BR |
| Publisher | Hindawi | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Aeromonas hydrophila | pt_BR |
| Subject in Portuguese | Genes associados | pt_BR |
| Subject in Portuguese | Virulência | pt_BR |
| Subject in Portuguese | Antimicrobianos | pt_BR |
| Subject in Portuguese | Animais | pt_BR |
| Subject in Portuguese | Alimentos | pt_BR |
| Subject in Portuguese | Humanos | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Virulence associated genes and Antimicrobial Resistance of Aeromonas hydrophila isolates from Animal, Food and Human Sources in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1155/2020/1052607 | |
| Abstract | Aeromonads are natural inhabitants of aquatic environments and may be associated with various human or animal diseases. Its pathogenicity is complex and multifactorial and is associated with many virulence factors. In this study, 110 selected Aeromonas hydrophila isolates isolated from food, animals, and human clinical material from 2010 to 2015 were analyzed. Antimicrobial susceptibility testing was performed by the disk diffusion method, and polymerase chain reaction was conducted to investigate the virulence genes hemolysin (hlyA), cytotoxic enterotoxin (act), heat-labile cytotonic enterotoxin (alt), aerolysin (aerA), and DNase-nuclease (exu). At least 92.7% of the isolates had one of the investigated virulence genes. Twenty different virulence profiles among the isolates were recognized, and the five investigated virulence genes were observed in four isolates. Human source isolates showed greater diversity than food and animal sources. Antimicrobial resistance was observed in 46.4% of the isolates, and multidrug resistance was detected in 3.6% of the isolates. Among the 120 isolates, 45% were resistant to cefoxitin; 23.5% to nalidixic acid; 16.6% to tetracycline; 13.7% to cefotaxime and imipenem; 11.8% to ceftazidime; 5.9% to amikacin, gentamicin, and sulfamethoxazole-trimethoprim; and 3.9% to ciprofloxacin and nitrofurantoin. Overall, the findings of our study indicated the presence of virulence genes and that antimicrobial resistance in A. hydrophila isolates in this study is compatible with potentially pathogenic bacteria. This information will allow us to recognize the potential risk through circulating isolates in animal health and public health and the spread through the food chain offering subsidies for appropriate sanitary actions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional de Referência em Diagnóstico de Enterobactérias. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Departamento de Tecnologia de Alimentos. Programa de Pós-Graduação em Higiene Veterinária e Processamento de Alimentos de Origem Animal. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional de Referência em Diagnóstico de Enterobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós-Graduação em Meio Ambiente e Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional de Referência em Diagnóstico de Enterobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós-Graduação em Meio Ambiente e Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Coordenação Geral dos Laboratórios de Sáude Pública. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Departamento de Tecnologia de Alimentos. Programa de Pós-Graduação em Higiene Veterinária e Processamento de Alimentos de Origem Animal. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional de Referência em Diagnóstico de Enterobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Departamento de Tecnologia de Alimentos. Programa de Pós-Graduação em Higiene Veterinária e Processamento de Alimentos de Origem Animal. Niterói, RJ, Brasil. | pt_BR |
| Subject | Aeromonas hydrophila | pt_BR |
| Subject | Antimicrobail Resistance | pt_BR |
| Subject | Virulence | pt_BR |
| Subject | Brazil | pt_BR |
| Subject | Food | pt_BR |
| Subject | Animal | pt_BR |
| Subject | Human | pt_BR |
| Subject | Associated Genes | pt_BR |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
