| Author | Manta, Fernanda S. N. | |
| Author | Barbieri, Raquel R. | |
| Author | Moreira, Suelen J. M. | |
| Author | Santos, Paulo T. S. | |
| Author | Nery, José A. C. | |
| Author | Duppre, Nádia C. | |
| Author | Sales, Anna M. | |
| Author | Pacheco, Antonio G. | |
| Author | Hacker, Mariana A. | |
| Author | Machado, Alice M. | |
| Author | Sarno, Euzenir N. | |
| Author | Moraes, Milton Ozório | |
| Access date | 2020-12-22T18:26:15Z | |
| Available date | 2020-12-22T18:26:15Z | |
| Document date | 2019 | |
| Citation | MANTA, Fernanda S. N. et al. Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study. 2011-2018. Scientific Reports, v. 9, p. 1-8, 2019. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45079 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | PCR Quantitativo | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Monitoramento | pt_BR |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Contatos familiares | pt_BR |
| Subject in Portuguese | Estudos de acompanhamento | pt_BR |
| Title | Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018 | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-019-52640-5 | |
| Abstract | Household contacts (HHC) of leprosy patients exhibit high-risk of developing leprosy and contact tracing is helpful for early diagnosis. From 2011 to 2018,2,437 HHC were examined in a clinic in Rio de Janeiro, Brazil and 16S qPCR was used for diagnosis and monitoring of contacts. Fifty-four HHCs were clinically diagnosed with leprosy at intake. Another 25 exhibited leprosy-like skin lesions at intake, 8 of which were confirmed as having leprosy (50% of which were qPCR positive) and 17 of which were diagnosed with other skin diseases (6% qPCR positive). In skin biopsies, qPCR presented a sensitivity of 0.50 and specificity of 0.94. Furthermore, 955 healthy HHCs were followed-up for at least 3 years and skin scrapings were collected from earlobes for qPCR detection. Positive qPCR indicated a non-significant relative risk of 2.52 of developing the disease. During follow-up, those who progressed towards leprosy exhibited 20% qPCR positivity, compared to 9% of those who remained healthy. Disease-free survival rates indicated that age had a significant impact on disease progression, where patients over 60 had a greater chance of developing leprosy [HR = 32.4 (3.6–290.3)]. Contact tracing combined with qPCR may assist in early diagnosis and age is a risk factor for leprosy progression. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Quantitative PCR | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Monitoring | pt_BR |
| Subject | Household contacts | pt_BR |
| Subject | Follow-up studies | pt_BR |
