| Author | Domingues, Carolina Salles | |
| Author | Cardoso, Flávia de Oliveira | |
| Author | Hardoim, Daiana de Jesus | |
| Author | Pelajo-Machado, Marcelo | |
| Author | Berto, Alvaro Luiz | |
| Author | Calabrese, Kátia da Silva | |
| Access date | 2020-12-30T18:03:17Z | |
| Available date | 2020-12-30T18:03:17Z | |
| Document date | 2020 | |
| Citation | DOMINGUES, Carolina Salles et al. Host Genetics Background Influence in the Intragastric Trypanosoma cruzi Infection. Frontiers in Immunology, v. 11, Article 566476, p. 1-20, Nov. 2020. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45478 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Ratos de raça | pt_BR |
| Subject in Portuguese | Infecção intragástrica | pt_BR |
| Subject in Portuguese | Formação genética do hospedeiro | pt_BR |
| Subject in Portuguese | Citocinas proinflamatórias | pt_BR |
| Subject in Portuguese | Linfócitos CD8+ | pt_BR |
| Title | Host Genetics Background Influence in the Intragastric Trypanosoma cruzi Infection | pt_BR |
| Type | Article | |
| DOI | 10.3389/fimmu.2020.566476 | |
| Abstract | Background: Considering the complexity of the factors involved in the immunopathology of Chagas disease, which influence the Chagas’ disease pathogenesis, anti-T. cruzi immune response, and chemotherapy outcome, further studies are needed to improve our understanding about these relationships. On this way, in this article we analyzed the host genetic influence on hematological, histopathological and immunological aspects after T. cruzi infection. Methods: BALB/c and A mice were intragastrically infected with T. cruzi SC2005 strain, isolated from a patient of an outbreak of Chagas disease. Parameters such as parasite load, survival rates, cytokines production, macrophages, T and B cell frequencies, and histopathology analysis were carried out. Results: BALB/c mice presented higher parasitemia and mortality rates than A mice. Both mouse lineages exhibited hematological alterations suggestive of microcytic hypochromic anemia and histopathological alterations in stomach, heart and liver. The increase of CD8+ T cells, in heart, liver and blood, and the increase of CD19+ B cells, in liver, associated with a high level of proinflammatory cytokines (IL-6, TNF-a, IFN-g), confer a resistance profile to the host. Although BALB/c animals exhibited the same findings observed in A mice, the response to infection occurred later, after a considerable parasitemia increase. By developing an early response to the infection, A mice were found to be less susceptible to T. cruzi SC2005 infection. Conclusions: Host genetics background shaping the response to infection. The early development of a cytotoxic cellular response profile with the production of proinflammatory cytokines is important to lead a less severe manifestation of Chagas disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Inbred mice | pt_BR |
| Subject | Intragastric infection | pt_BR |
| Subject | Host genetic background | pt_BR |
| Subject | Proinflammatory cytokines | pt_BR |
| Subject | CD8+ lymphocytes | pt_BR |
