| Author | Macedo, Isabel S. | |
| Author | Lima, Marcos V. A. | |
| Author | Souza, Jéssica S. | |
| Author | Rochael, Natalia C. | |
| Author | Caldas, Pedro N. | |
| Author | Barbosa, Helene S. | |
| Author | Lara, Flávio A. | |
| Author | Saraiva, Elvira M. | |
| Author | Mariante, Rafael M. | |
| Access date | 2021-01-06T19:31:20Z | |
| Available date | 2021-01-06T19:31:20Z | |
| Document date | 2020 | |
| Citation | MACEDO, Isabela S. et al. Extracellular Traps Released by Neutrophils from Cats are Detrimental to Toxoplasma gondii Infectivity. Microorganisms, v.8, 1628, 18p, 2020. | pt_BR |
| ISSN | 2076-2607 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45517 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Cálcio | pt_BR |
| Subject in Portuguese | Toxoplasma gondii | pt_BR |
| Subject in Portuguese | Anfitrião definitivo | pt_BR |
| Subject in Portuguese | Elastase | pt_BR |
| Subject in Portuguese | Infetividade | pt_BR |
| Subject in Portuguese | Armadilha extracelular de neutrófilo | pt_BR |
| Subject in Portuguese | Imagem ao vivo | pt_BR |
| Subject in Portuguese | PI3K | pt_BR |
| Title | Extracellular Traps Released by Neutrophils from Cats are Detrimental to Toxoplasma gondii Infectivity | pt_BR |
| Type | Article | |
| DOI | 10.3390/microorganisms8111628 | |
| Abstract | Abstract: Toxoplasma gondii is the causative agent of toxoplasmosis, an infectious disease that a ects
over 30% of the human world population, causing fatal infections in immunocompromised individuals
and neonates. The life cycle of T. gondii is complex, and involves intermediate hosts (birds and
mammals) and definitive hosts (felines, including domestic cats). The innate immune repertoire
against the parasite involves the production of neutrophil extracellular traps (NET), and neutrophils
from several intermediate hosts produce NET induced by T. gondii. However, the mechanisms
underlying NET release in response to the parasite have been poorly explored. Therefore, the aims of
this study were to investigate whether neutrophils from cats produce NET triggered by T. gondii and
to understand the mechanisms thereby involved. Neutrophils from cats were stimulated with T. gondii
tachyzoites and NET-derived DNA in the supernatant was quantified during the time. The presence
of histone H1 and myeloperoxidase was detected by immunofluorescence. We observed that cat
neutrophils produce both classical and rapid/early NET stimulated by T. gondii. Inhibition of elastase,
intracellular calcium, and phosphatidylinositol 3-kinase (PI3K)- partially blocked classical NET
release in response to the parasite. Electron microscopy revealed strands and networks of DNA in
close contact or completely entrapping parasites. Live imaging showed that tachyzoites are killed by
NET. We conclude that the production of NET is a conserved strategy to control infection by T. gondii
amongst intermediate and definitive hosts. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | HVN Hospital Veterinário Niterói. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Calcium | pt_BR |
| Subject | Definitive host | pt_BR |
| Subject | Elastase | pt_BR |
| Subject | Felid | pt_BR |
| Subject | Unfectivity | pt_BR |
| Subject | Klling | pt_BR |
| Subject | Live imaging | pt_BR |
| Subject | Neutrophil extracellular trap | pt_BR |
| Subject | PI3K | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
