| Author | Cavalcante, Bruno R. R. | |
| Author | França, Luciana S. Aragão | |
| Author | Sampaio, Gabriela L. A. | |
| Author | Nonaka, Carolina K. V. | |
| Author | Oliveira, Moisés S. | |
| Author | Campos, Gúbio S. | |
| Author | Sardi, Silvia I. | |
| Author | Dias, Beatriz Rocha Simões | |
| Author | Menezes, Juliana Perrone Bezerra de. | |
| Author | Rocha, Vinícius P. C. | |
| Author | Rossi, Erik A. | |
| Author | Paredes, Bruno D. | |
| Author | Martins, Gabriele L. S. | |
| Author | Allahdadi, Kyan J. | |
| Author | Peixoto, Laisla R. | |
| Author | Barbosa Filho, José M. | |
| Author | Souza, Bruno S. F. | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2021-01-08T11:20:03Z | |
| Available date | 2021-01-08T11:20:03Z | |
| Document date | 2020 | |
| Citation | CAVALCANTE, Bruno R. R. et al. Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells. Frontiers in Cellular and Infection Microbiology, p. 1-13, 2020. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45538 | |
| Description | 1 Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, Brazil, 2 D’Or Institute for Research and
Education (IDOR), Rio de Janeiro, Brazil, 3 Gonc¸alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil,
4 Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil, 5 Department of Pharmacy, Federal University of
Paraíba, João Pessoa, Brazil | pt_BR |
| Sponsorship | Brazilian National Council for
Scientific and Technological Development (CNPq), DECIT/MS
(grant number 443909/2018-0), INCT-REGENERA (grant
number 465656/2014‐5) and the Coordination for the
Improvement of Higher Education Personnel (CAPES/Zika
Fast track). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Zika vírus | pt_BR |
| Subject in Portuguese | Células progenitoras | pt_BR |
| Subject in Portuguese | Ácido betulínico | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Subject in Portuguese | Neuroproteção | pt_BR |
| Title | Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fcimb.2020.558324 | |
| Abstract | Zika virus (ZIKV), a member of the Flaviviridae family, was brought into the spotlight due to its widespread and increased pathogenicity, including Guillain-Barré syndrome and microcephaly. Neural progenitor cells (NPCs), which are multipotent cells capable of differentiating into the major neural phenotypes, are very susceptible to ZIKV infection. Given the complications of ZIKV infection and potential harm to public health, effective treatment options are urgently needed. Betulinic acid (BA), an abundant terpenoid of the lupane group, displays several biological activities, including neuroprotective effects. Here we demonstrate that Sox2+ NPCs, which are highly susceptible to ZIKV when compared to their neuronal counterparts, are protected against ZIKV-induced cell death when treated with BA. Similarly, the population of Sox2+ and Casp3+ NPCs found in ZIKVinfected cerebral organoids was significantly higher in the presence of BA than in untreated controls. Moreover, well-preserved structures were found in BA-treated organoids in contrast to ZIKV-infected controls. Bioinformatics analysis indicated Akt pathway activation by BA treatment. This was confirmed by phosphorylated Akt analysis, both in BA-treated NPCs and brain organoids, as shown by immunoblotting and immunofluorescence analyses, respectively. Taken together, these data suggest a neuroprotective role of BA in ZIKV-infected NPCs. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Centro de Ciências da Saúde. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Centro de Ciências da Saúde. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Subject | Zika virus | pt_BR |
| Subject | Neural progenitor cells | pt_BR |
| Subject | Betulinic acid | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Neuroprotection | pt_BR |
