| Author | Pereira, Miriam Salles | |
| Author | Cardoso, Liana Monteiro da Fonseca | |
| Author | Silva, Tatiane Barreto da | |
| Author | Teixeira, Ayla Josma | |
| Author | Mizrahi, Saul Eliahú | |
| Author | Ferreira, Gabriel Schonwandt Mendes | |
| Author | Dantas, Fabio Moyses Lins | |
| Author | Almeida, Vinicius Cotta de | |
| Author | Alves, Luiz Anastacio | |
| Access date | 2021-01-10T15:15:56Z | |
| Available date | 2021-01-10T15:15:56Z | |
| Document date | 2020 | |
| Citation | PEREIRA, Miriam Salles et al. A Low-Cost Open Source Device for Cell Microencapsulation. Materials, v. 13, p. 1-14, Nov. 2020. | pt_BR |
| ISSN | 1996-1944 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45549 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Alginato | pt_BR |
| Subject in Portuguese | Impressora 3D | pt_BR |
| Subject in Portuguese | Microencapsulação | pt_BR |
| Subject in Portuguese | Transplante celular | pt_BR |
| Subject in Portuguese | Terapia celular | pt_BR |
| Title | A Low-Cost Open Source Device for Cell Microencapsulation | pt_BR |
| Type | Article | |
| DOI | 10.3390/ma13225090 | |
| Abstract | Microencapsulation is a widely studied cell therapy and tissue bioengineering technique, since it is capable of creating an immune-privileged site, protecting encapsulated cells from the host immune system. Several polymers have been tested, but sodium alginate is in widespread use for cell encapsulation applications, due to its low toxicity and easy manipulation. Di erent cell encapsulation methods have been described in the literature using pressure di erences or electrostatic changes with high cost commercial devices (about 30,000 US dollars). Herein, a low-cost device (about 100 US dollars) that can be created by commercial syringes or 3D printer devices has been developed. The capsules, whose diameter is around 500 mand can decrease or increase according to the pressure applied to the system, is able to maintain cells viable and functional. The hydrogel porosity of the capsule indicates that the immune system is not capable of destroying host cells, demonstrating that new studies can be developed for cell therapy at low cost with microencapsulation production. This device may aid pre-clinical and clinical projects in low- and middle-income countries and is lined up with open source equipment devices. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil / Centro Universitário de Volta Redonda. UniFOA. Volta Redonda, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Tecnologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Tecnologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Tecnologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Alginate | pt_BR |
| Subject | 3D printer | pt_BR |
| Subject | Microencapsulation | pt_BR |
| Subject | Cell transplantation | pt_BR |
| Subject | Cell therapy | pt_BR |
