| Author | Mojoli, Andrés | |
| Author | Gonçalves, Barbara Simonson | |
| Author | Temerozo, Jairo R. | |
| Author | Alves, Bruno Cister | |
| Author | Geddes, Victor | |
| Author | Herlinger, Alice | |
| Author | Aguiar, Renato Santana | |
| Author | Pilotto, José Henrique | |
| Author | Saraiva, Elvira M. | |
| Author | Bou‑Habib, Dumith Chequer Bou | |
| Access date | 2021-01-10T18:06:12Z | |
| Available date | 2021-01-10T18:06:12Z | |
| Document date | 2020 | |
| Citation | MOJOLI, Andrés et al. Neutrophil extracellular traps from healthy donors and HIV‑1‑infected individuals restrict HIV‑1 production in macrophages. Scientifc Reports, v. 10, p. 1-15, 2020. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45550 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Neutrófilos extracelulares | pt_BR |
| Subject in Portuguese | Armadilhas | pt_BR |
| Subject in Portuguese | Doadores saudáveis | pt_BR |
| Subject in Portuguese | indivíduos infectados | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Produção de HIV-1 | pt_BR |
| Title | Neutrophil extracellular traps from healthy donors and HIV‑1‑infected individuals restrict HIV‑1 production in macrophages | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-020-75357-2 | |
| Abstract | Neutrophils release extracellular traps (NETs) after interaction with microorganisms and physiological
or synthetic products. NETs consist of decondensed chromatin complexed with proteins, some of
them with microbicidal properties. Because NETs can modulate the functioning of HIV-1 target cells,
we aimed to verify whether they modify HIV-1 replication in macrophages. We found that exposure
of HIV-1-infected macrophages to NETs resulted in significant inhibition of viral replication. The NET
anti-HIV-1 action was independent of other soluble factors released by the activated neutrophils,
but otherwise dependent on the molecular integrity of NETs, since NET-treatment with protease or
DNase abolished this effect. NETs induced macrophage production of the anti-HIV-1 β-chemokines
Rantes and MIP-1β, and reduced the levels of integrated HIV-1 DNA in the macrophage genome,
which may explain the decreased virus production by infected macrophages. Moreover, the residual
virions released by NET-treated HIV-1-infected macrophages lost infectivity. In addition, elevated
levels of DNA-elastase complexes were detected in the plasma from HIV-1-infected individuals,
and neutrophils from these patients released NETs, which also inhibited HIV-1 replication in in vitro
infected macrophages. Our results reveal that NETs may function as an innate immunity mechanism
able to restrain HIV-1 production in macrophages. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microniologia Prof. Paulo de Góes, Departamento de Imunologia. Laboratório de Imunobiologia da Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Neutrophil extracellular | pt_BR |
| Subject | Traps | pt_BR |
| Subject | Healthy donors | pt_BR |
| Subject | HIV‑1‑infected individuals | pt_BR |
| Subject | restrict HIV‑1 production | pt_BR |
| Subject | Macrophages | pt_BR |
| e-ISSN | 2045-2322 | |
