| Author | Legros, Vincent | |
| Author | Jeannin, Patricia | |
| Author | Burlaud-Gaillard, Julien | |
| Author | Chaze, Thibault | |
| Author | Gianetto, Quentin Giai | |
| Author | Butler-Brown, Gillian | |
| Author | Mouly, Vincent | |
| Author | Zoladek, Jim | |
| Author | Afonso, Philippe V. | |
| Author | Gonzalez, Mariela Natacha | |
| Author | Matondo, Mariette | |
| Author | Riederer, Ingo | |
| Author | Roingeard, Philippe | |
| Author | Gessain, Antoine | |
| Author | Choumet, Valérie | |
| Author | Emmanuel Ceccaldi, Pierre | |
| Access date | 2021-01-10T19:22:21Z | |
| Available date | 2021-01-10T19:22:21Z | |
| Document date | 2020 | |
| Citation | LEGROS, Vincent et al. Differentiation-dependent susceptibility of human muscle cells to Zika virus infection. PLoS Neglected Tropical Diseases, v. 14, n. 8, e0008282, p. 1-23, Aug. 2020. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45553 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Suscetibilidade dependente | pt_BR |
| Subject in Portuguese | Células musculares humanas | pt_BR |
| Subject in Portuguese | Zika vírus | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Title | Differentiation-dependent susceptibility of human muscle cells to Zika virus infection | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0008282 | |
| Abstract | Muscle cells are potential targets of many arboviruses, such as Ross River, Dengue, Sindbis,
and chikungunya viruses, that may be involved in the physiopathological course of the
infection. During the recent outbreak of Zika virus (ZIKV), myalgia was one of the most frequently
reported symptoms. We investigated the susceptibility of human muscle cells to
ZIKV infection. Using an in vitro model of human primary myoblasts that can be differentiated
into myotubes, we found that myoblasts can be productively infected by ZIKV. In contrast,
myotubes were shown to be resistant to ZIKV infection, suggesting a differentiationdependent
susceptibility. Infection was accompanied by a caspase-independent cytopathic
effect, associated with paraptosis-like cytoplasmic vacuolization. Proteomic profiling was
performed 24h and 48h post-infection in cells infected with two different isolates. Proteome
changes indicate that ZIKV infection induces an upregulation of proteins involved in the activation
of the Interferon type I pathway, and a downregulation of protein synthesis. This work
constitutes the first observation of primary human muscle cells susceptibility to ZIKV infection,
and differentiation-dependent restriction of infection from myoblasts to myotubes.
Since myoblasts constitute the reservoir of stem cells involved in reparation/regeneration in
muscle tissue, the infection of muscle cells and the viral-induced alterations observed here
could have consequences in ZIKV infection pathogenesis. | pt_BR |
| Affilliation | Unité Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Institut Pasteur. Paris, France / Université de Paris. Paris, France / UMR CNRS 3569, Paris, France. | pt_BR |
| Affilliation | Unité Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Institut Pasteur. Paris, France / Université de Paris. Paris, France / UMR CNRS 3569, Paris, France. | pt_BR |
| Affilliation | INSERM U1259 & Plate Forme IBiSA de Microscopie Electronique, Université François Rabelais and CHRU, Tours, France. | pt_BR |
| Affilliation | Proteomics Platform, Mass Spectrometry for Biology Unit, USR 2000 IP CNRS.Institut Pasteur, Paris, France. | pt_BR |
| Affilliation | Proteomics Platform, Mass Spectrometry for Biology Unit, USR 2000 IP CNRS.Institut Pasteur, Paris, France / Bioinformatics and Biostatistics Hub, C3BI, USR 3756 IP CNRS, Institut Pasteur, Paris, France. | pt_BR |
| Affilliation | Sorbonne Université. Institut National de la Santé et de la Recherche Médicale, Association Institut de Myologie, Centre de Recherche en Myologie, UMRS974, Paris, France. | pt_BR |
| Affilliation | Sorbonne Université. Institut National de la Santé et de la Recherche Médicale, Association Institut de Myologie, Centre de Recherche en Myologie, UMRS974, Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Proteomics Platform, Mass Spectrometry for Biology Unit, USR 2000 IP CNRS.Institut Pasteur, Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | INSERM U1259 & Plate Forme IBiSA de Microscopie Electronique, Université François Rabelais and CHRU, Tours, France. | pt_BR |
| Affilliation | Unité Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Institut Pasteur. Paris, France / Université de Paris. Paris, France / UMR CNRS 3569, Paris, France. | pt_BR |
| Affilliation | Unité Environnement et Risques Infectieux, Département de Santé Globale, Institut Pasteur, Paris, France | pt_BR |
| Affilliation | Unité Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Institut Pasteur. Paris, France / Université de Paris. Paris, France / UMR CNRS 3569, Paris, France. | pt_BR |
| Subject | Dependent susceptibility | pt_BR |
| Subject | Human muscle cells | pt_BR |
| Subject | Zika virus | pt_BR |
| Subject | Infection | pt_BR |
