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AutorSilva, Jéssica Rebouças
AutorTadini, Maraine Catarina
AutorNunes, Danielle Devequi
AutorMansur, Ana Luíza
AutorMattos, Paulo Sérgio de Morais da Silveira
AutorOliveira, Camila Indiani de
AutorFormiga, Fabio Rocha
AutorBerretta, Andresa A
AutorOliveira, Franciane Marquele
AutorBorges, Valéria de Matos
Fecha de acceso2021-01-27T13:21:07Z
Fecha de disponibilización2021-01-27T13:21:07Z
Fecha de publicación2020
ReferenciaSILVA, Jéssica Rebouças et al. Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection. International Journal of Nanomedicine, v. 15, p. 8659–8672, 2020.pt_BR
ISSN1176-9114pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/45839
PromociónFIOCRUZ (VMB), FAPESP Grant fellowships (2015/ 15948-5, 2016/10145-4, 2016/10345-3, 2016/11116-8 and 2017/22888-4) (FMO) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. AAB, CIO and VMB are senior scientist fellows at the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). JRS is supported by a scholarship from CAPES. PSSM holds a fellowship from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).pt_BR
Idiomaengpt_BR
EditorDove Medical Presspt_BR
Derechos de autoropen accesspt_BR
Palabras clave en PortuguésLeishmaniosept_BR
Palabras clave en PortuguésDoenças negligenciadaspt_BR
Palabras clave en PortuguésNanopartículaspt_BR
Palabras clave en PortuguésAdministração de drogaspt_BR
TítuloEvaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infectionpt_BR
Tipo del documentoArticlept_BR
Resumen en InglésLeishmaniasis is a neglected disease, and the current therapeutic arsenal for its treatment is seriously limited by high cost and toxicity. Nanostructured lipid carriers (NLCs) represent a promising approach due to high drug loading capacity, controlled drug release profiles and superior stability. Here, we explore the efficacy of a unique pH-sensitive amphotericin B-loaded NLC (AmB-NLC) in Leishmania braziliensis infection in vitro and in vivo. Methods and Results: AmB-NLC was assessed by dynamic light scattering and atomic force microscopy assays. The carrier showed a spherical shape with a nanometric size of 242.0 ± 18.3 nm. Zeta potential was suggestive of high carrier stability (−42.5 ± 1.5 mV), and the NLC showed ~99% drug encapsulation efficiency (EE%). In biological assays, AmB-NLC presented a similar IC50 as free AmB and conventional AmB deoxycholate (AmB-D) (11.7 ± 1.73; 5.3 ± 0.55 and 13 ± 0.57 ng/mL, respectively), while also presenting higher selectivity index and lower toxicity to host cells, with no observed production of nitric oxide or TNF-α by in vitro assay. Confocal microscopy revealed the rapid uptake of AmBNLC by infected macrophages after 1h, which, in association with more rapid disruption of AmB-NLC at acidic pH levels, may directly affect intracellular parasites. Leishmanicidal effects were evaluated in vivo in BALB/c mice infected in the ear dermis with L. braziliensis and treated with a pentavalent antimonial (Sb5+), liposomal AmB (AmB-L) or AmB-NLC. After 6 weeks of infection, AmB-NLC treatment resulted in smaller ear lesion size in all treated mice, indicating the efficacy of the novel formulation. Conclusion: Here, we preliminarily demonstrate the effectiveness of an innovative and cost-effective AmB-NLC formulation in promoting the killing of intracellular L. braziliensis. This novel carrier system could be a promising alternative for the future treatment of cutaneous leishmaniasis.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Bahia, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.pt_BR
AfiliaciónEleve Science Research and Development. Ribeirão Preto, SP, Brazil / University of São Paulo. Faculty of Philosophy, Sciences and Letters of Ribeirão Preto. Department of Chemistry. Ribeirão Preto, SP, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Bahia, Brasil / Institute of Innovation in Advanced Health Systems. Laboratory of Pharmaceutical Formulations. Salvador, BA, Brazil;.pt_BR
AfiliaciónEleve Science Research and Development. Ribeirão Preto, SP, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Bahia, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.pt_BR
AfiliaciónFederal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Vector- Doenças Infecciosas Transmitidas. Salvador, BA, Bahia, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Pernambuco. Postgraduate Program in Applied Cellular and Molecular Biology. Recife, PE, Brazil.pt_BR
AfiliaciónApis Flora Industrial e Comercial Ltda. Laboratory of Research, Development and Innovation. Ribeirão Preto, SP, Brazil.pt_BR
AfiliaciónEleve Science Research and Development. Ribeirão Preto, SP, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Bahia, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.pt_BR
Palavras clave en InglêsLeishmaniasispt_BR
Palavras clave en InglêsNeglected diseasept_BR
Palavras clave en InglêsNanoparticlespt_BR
Palavras clave en InglêsDrug deliverypt_BR


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