| Author | Vilela, Felipe Pinheiro | |
| Author | Gomes, Carolina Nogueira | |
| Author | Paziani, Mário Henrique | |
| Author | Braz, Vânia Santos | |
| Author | Rodrigues, Dália dos Prazeres | |
| Author | Costa, Renata Garcia | |
| Author | Tiba-Casas, Monique Ribeiro | |
| Author | von Zeska Kress, Márcia Regina | |
| Author | Falcão, Juliana Pfrimer | |
| Author | Campioni, Fábio | |
| Access date | 2021-01-29T19:35:33Z | |
| Available date | 2021-01-29T19:35:33Z | |
| Document date | 2020 | |
| Citation | VILELA, Felipe Pinheiro et al. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. Infection, Genetics and Evolution, v. 80, 104193, p. 1-9, Jan. 2020. | pt_BR |
| ISSN | 1567-1348 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45882 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Salmonella Dublin | pt_BR |
| Subject in Portuguese | RT-PCR | pt_BR |
| Subject in Portuguese | Galleria mellonella | pt_BR |
| Subject in Portuguese | Invasão celular | pt_BR |
| Subject in Portuguese | ST313 | pt_BR |
| Title | Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.meegid.2020.104193 | |
| Abstract | Salmonella Dublin is a strongly cattle-adapted serovar that has also been responsible for severe invasive infections
in humans. Although invasive infections by non-typhoid Salmonella have increased in developed and in
developing countries, in sub-Saharan Africa these infections have been frequently related to Salmonella
Typhimurium strains from Sequence Type (ST) 313 that harbor a possible virulence marker, the bstA gene,
broadly detected in S. Dublin strains. The aims of this study were to verify the frequency of bstA by PCR in 113
Salmonella Dublin strains isolated from humans (83) and animals (30) in Brazil and the expression by RT-PCR of
bstA, sopE2 and fliC in six strains isolated from humans (4) and animals (2). Moreover, the invasion capacity in
Caco-2 human epithelial cells and U937 human macrophages, plus in vivo virulence analysis in Galleria mellonella
and the motility were verified for 20 S. Dublin strains isolated from humans (15) and animals (5). All studied
strains presented the bstA gene. The relative expression rates ranged from 0.1 to 2.3 fold change for bstA and
from no expression to 16.6 fold change for sopE2, while no expression was detected for fliC. The invasion in
Caco-2 cells ranged from 54.0 to 88.9% and in U937 cells from 72.9 to 98.1% in the 20 strains studied. In
addition, 17 strains presented a highly virulent profile in the G. mellonella model and 15 strains presented a nonmotile
profile. In conclusion, the presence and expression of bstA in the S. Dublin strains studied suggested that
this gene may influence in the invasive characteristic of this serovar. The low expression of sopE2 in strains from
human invasive cases suggested that its expression may not be a limiting factor to the invasion of S. Dublin
strains. The absence of fliC expression and the low motility rates observed suggest that the flagella absence may
favor the host immune system evasion by S. Dublin and the establishment of infection. Moreover, the high
mortality rates observed in vivo in Galleria mellonella reinforce the pathogenic potential of S. Dublin strains. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de São Paulo. Faculdade de Ciências Farmacêuticas - Câmpus de Araraquara. Araraquara, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Adolfo Lutz. Centro de Bacteriologia, São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Subject | Salmonella Dublin | pt_BR |
| Subject | Cell invasion | pt_BR |
| Subject | Galleria mellonella | pt_BR |
| Subject | ST313 | pt_BR |
| Subject | RT-PCR | pt_BR |
