Author | Duarte, Cristiane Angélico | |
Author | Goulart, Luiz Ricardo | |
Author | Filice, Letícia de Souza Castro | |
Author | Lima, Isabela Lemos de | |
Author | Fernández, Esther Campos | |
Author | Dantas, Noelio Oliveira | |
Author | Silva, Anielle Christine Almeida | |
Author | Soares, Milena Botelho Pereira | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Cardoso, Carine Machado Azevedo | |
Author | França, Luciana Souza de Aragão | |
Author | Rocha, Vinícius Pinto Costa | |
Author | Ribeiro, Ana Rosa Lopes Pereira | |
Author | Perez, Geronimo | |
Author | Carvalho, Loyna Nobile | |
Author | Goulart, Vivian Alonso | |
Access date | 2021-02-05T11:45:39Z | |
Available date | 2021-02-05T11:45:39Z | |
Document date | 2020 | |
Citation | DUARTE, Cristiane Angélico et al. Characterization of Crystalline Phase of TiO2 Nanocrystals, Cytotoxicity and Cell Internalization Analysis on Human Adipose Tissue-Derived Mesenchymal Stem Cells. Materials, v. 13, p. 1-16, 2020. | pt_BR |
ISSN | 1996-1944 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/45971 | |
Description | Milena Botelho Pereira Soares; Ricardo Ribeiro dos Santos; Carine Machado Azevedo Cardoso; Luciana Souza de Aragão França; Vinícius Pinto Costa Rocha. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Description | 1Laboratory of Nanobiotechnology, Institute of Biotechnology (IBTEC), Federal University of Uberlandia, Uberlandia 38.400-000, Brazil; cris.angelico@terra.com.br (C.A.D.); goulartlr@gmail.com (L.R.G.); leticiafilice@ufu.br (L.d.S.C.F.); isabela.lemos@hotmail.com (I.L.d.L.); esther.fernandez@ufu.br (E.C.-F.); loyna.nobile@gmail.com (L.N.C.) 2 Laboratory of New Nanostructured and Functional Materials, Physics Institute, Federal University of Alagoas, Maceió 57073-704, Brazil; noelio@fis.ufal.br (N.O.D.); aniellechristineas@gmail.com (A.C.A.S.) 3 Laboratory of Tissue Engineering and Immunopharmacology (LETI), Oswaldo Cruz Fundation, Salvador 40.000-001, Brazil; milenabpsoares@gmail.com (M.B.P.S.); ricardoribeiro1941@gmail.com (R.R.d.S.); cariazevedo@hotmail.com (C.M.A.C.); luaragao@gmail.com (L.S.d.A.F.); vinyrocha@hotmail.com (V.P.C.R.) 4 Center of Biotechnology and Cell Therapy (CBTC), Sao Rafael Hospital, Salvador 40.000-001, Brazil 5 National Institute of Metrology, Quality and Technology (INMETRO), Institute of Biomedical Engineering, Rio de Janeiro 20.000-000, Brazil; analopes0781@gmail.com 6 Brazilian Branch of Institute of Biomaterials, Tribocorrosion and Nanomedicine (IBTN), Rio de Janeiro 20.000-000, Brazil 7 Division of Metrology of Materials, National Institute of Metrology, Quality and Technology (INMETRO), Rio de Janeiro 20.000-000, Brazil; perezgeronimo@hotmail.com 8 Laboratory of Nanotechnology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 20.000-000, Brazil * Correspondence: alonso.goulart@ufu.br; Tel.: +55-34-3235-8440
† Current Institution: Biomaterials, Biodegradables and Biomimetics Research Institute (I3Bs), University of Minho, 4800-058 Guimarães, Portugal. | pt_BR |
Sponsorship | CNPq, CAPES, and FAPEMIG which provided financial support
to the National Institute of Science and Technology in Theranostics and Nanobiotechnology (grant number
APQ-03613-17). | pt_BR |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Nanocristal | pt_BR |
Subject in Portuguese | Fase cristalina | pt_BR |
Subject in Portuguese | Célula-tronco mesenquimais do tecido adiposo | pt_BR |
Title | Characterization of Crystalline Phase of TiO2 Nanocrystals, Cytotoxicity and Cell Internalization Analysis on Human Adipose Tissue-Derived Mesenchymal Stem Cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/ma13184071 | |
Abstract | Titanium dioxide (TiO2) is manufactured worldwide as crystalline and amorphous forms for
multiple applications, including tissue engineering, but our study proposes analyzing the impact of
crystalline phases of TiO2 on Mesenchymal Stem Cells (MSCs). Several studies have already described
the regenerative potential of MSCs and TiO2 has been used for bone regeneration. In this study,
polydispersity index and sizes of TiO2 nanocrystals (NCs) were determined. Adipose tissue-derived
Mesenchymal Stem Cells (AT-MSCs) were isolated and characterized in order to evaluate cellular
viability and the internalization of nanocrystals (NCs). All of the assays were performed using the
TiO2 NCs with 100% anatase (A), 91.6% anatase/9.4% rutile (AR), 64.6% rutile/35.4% anatase (RA),
and 84.0% rutile/16% brookite (RB), submitted to several concentrations in 24-h treatments. Cellular
localization of TiO2 NCs in the AT-MSCs was resolved by europium-doped NCs. Viability was
significantly improved under the predominance of the rutile phase in NCs with localization restricted
at the cytoplasm, suggesting that AR and RA NCs are not genotoxic and can be associated with most
cellular activities and metabolic pathways, including glycolysis and cell division. | pt_BR |
Affilliation | Múltipla - Ver em Notas. | pt_BR |
Subject | TiO2 nanocrystal | pt_BR |
Subject | Crystalline phase | pt_BR |
Subject | Adipose tissue stem cell | pt_BR |