| Author | Nascimento, Ana Paula Barbosa do | |
| Author | Medeiros Filho, Fernando | |
| Author | Pauer, Heidi | |
| Author | Antunes, Luis Caetano Martha | |
| Author | Sousa, Hério | |
| Author | Senger, Hermes | |
| Author | Albano, Rodolpho Mattos | |
| Author | Santos, Marcelo Trindade dos | |
| Author | Carvalho-Assef, Ana Paula D’Alincourt | |
| Author | Silva, Fabrício Alves Barbosa da | |
| Access date | 2021-02-11T19:05:10Z | |
| Available date | 2021-02-11T19:05:10Z | |
| Document date | 2020 | |
| Citation | NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46046 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | SPM-1 | pt_BR |
| Subject in Portuguese | Produtoras de metalo-beta-lactamase | pt_BR |
| Subject in Portuguese | Genômica comparativa | pt_BR |
| Subject in Portuguese | Análise fenotípica | pt_BR |
| Title | Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-020-69944-6 | |
| Abstract | Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated
infections. The Brazilian isolate, named CCBH4851, is a multidrug-resistant clone belonging to the
sequence type 277. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated
with the presence of the blaSPM-1 gene, encoding a metallo-beta-lactamase, in combination with other
exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are
essential to identify key features of their physiology that may lead to the identification of new targets
for therapy. Using both Illumina and PacBio sequencing data, we obtained a single contig representing
the CCBH4851 genome with annotated features that were consistent with data reported for the
species. However, comparative analysis with other Pseudomonas aeruginosa strains revealed genomic
differences regarding virulence factors and regulatory proteins. In addition, we performed phenotypic
assays that revealed CCBH4851 is impaired in bacterial motilities and biofilm formation. On the other
hand, CCBH4851 genome contained acquired genomic islands that carry transcriptional factors,
virulence and antimicrobial resistance-related genes. Presence of single nucleotide polymorphisms in
the core genome, mainly those located in resistance-associated genes, suggests that these mutations
may also influence the multidrug-resistant behavior of CCBH4851. Overall, characterization of
Pseudomonas aeruginosa CCBH4851 complete genome revealed the presence of features that strongly
relates to the virulence and antibiotic resistance profile of this important infectious agent. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz; Escola Nacional de Saúde Pùblica Sergio Arouca. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | SPM‑1 | pt_BR |
| Subject | Metallo‑beta‑lactamase‑producing | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | Comparative genomics | pt_BR |
| Subject | Phenotypic analysis | pt_BR |
