| Author | Santos, Gisele Graça Leite dos | |
| Author | Oliveira, Anna Lethícia Lima | |
| Author | Santos, Dourivaldo Silva | |
| Author | Espírito Santo, Renan Fernandes do | |
| Author | Silva, Daniela Nascimento | |
| Author | Juiz, Paulo José Lima | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Villarreala, Cristiane Flora | |
| Access date | 2021-03-05T13:45:34Z | |
| Available date | 2021-03-05T13:45:34Z | |
| Document date | 2020 | |
| Citation | SANTOS, Gisele Graça Leite dos et al. Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord. Life Sciences, p. 1-23, Nov. 2020. | |
| ISSN | 0024-3205 | |
| URI | https://www.arca.fiocruz.br/handle/icict/46270 | |
| Sponsorship | FAPESB [grant number RED0010/2012]. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Subject in Portuguese | Células Tronco Mesenquimais | pt_BR |
| Subject in Portuguese | Quimioterapia | pt_BR |
| Subject in Portuguese | Estresse oxidativo | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Title | Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord | en_US |
| Type | Preprint | |
| DOI | 10.1016/j.lfs.2020.118755 | |
| Abstract | The present study was designed to investigate whether the antinociceptive effect of bone marrow-derived mesenchymal stem/stromal cells (MSC) during oxaliplatin (OXL)-induced sensory neuropathy is related to antioxidant properties. Main methods: Male mice C57BL/6 were submitted to repeated intravenous administration of OXL (1 mg/kg, 9 administrations). After the establishment of sensory neuropathy, mice were treated with a single intravenous administration of MSC (1x106), vehicle or gabapentin. Paw mechanical and thermal nociceptive thresholds were evaluated through von Frey filaments and cold plate test, respectively. Motor performance was evaluated in the rotarod test. Gene expression profile, cytokine levels, and oxidative stress markers in the spinal cord were evaluated by real-time PCR, ELISA and biochemical assays, respectively. Key findings: OXL-treated mice presented behavioral signs of sensory neuropathy, such as mechanical allodynia and thermal hyperalgesia, which were completely reverted by a single administration of MSC. Repeated oral treatment with gabapentin (70 mg/kg) induced only transient antinociception. The IL-1β and TNF-α spinal levels did not differ between mice with or without sensory neuropathy. MSC increased the levels of anti-inflammatory cytokines, IL-10 and TGF-β, in the spinal cord of neuropathic mice, in addition to increasing the gene expression of antioxidant factors SOD and Nrf-2. Additionally, nitrite and MDA spinal levels were reduced by the MSC treatment. Significance: MSC induce reversion of sensory neuropathy induced by OXL possibly by activation of anti-inflammatory and antioxidant pathways, leading to reestablishment of redox homeostasis in the spinal cord. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | |
| Affilliation | Federal University of Bahia. College of Pharmacy. Salvador, BA, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | |
| Affilliation | University Center SENAI/CIMATEC. SENAI Institute of Innovation in Advanced Health Systems. Salvador, BA, Brazil. | |
| Affilliation | Federal University of Recôncavo of Bahia. Feira de Santana, BA, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / University Center SENAI/CIMATEC. SENAI Institute of Innovation in Advanced Health Systems. Salvador, BA, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Federal University of Bahia. College of Pharmacy. Salvador, BA, Brazil. | |
| Subject | Neuropathic pain | en_US |
| Subject | Chemotherapy | en_US |
| Subject | Mesenchymal Cells | en_US |
| Subject | Oxidative Stress | en_US |
| Subject | Cytokines | en_US |
| Embargo date | 2030-12-31 | |
