| Author | Rodríguez-Vega, Andrés | |
| Author | Losada-Barragán, Monica | |
| Author | Berbert, Luiz Ricardo | |
| Author | Rodrigues, Camila Mesquita | |
| Author | Bombaça, Ana Cristina Souza | |
| Author | Menna-Barreto, Rubem | |
| Author | Aquino, Priscila | |
| Author | Carvalho, Paulo C. | |
| Author | Padrón, Gabriel | |
| Author | Jesus, Jose Batista de | |
| Author | Cuervo, Patricia | |
| Access date | 2021-03-06T20:42:54Z | |
| Available date | 2021-03-06T20:42:54Z | |
| Document date | 2021 | |
| Citation | RODRÍGUEZ-VEGA, Andrés et al. Quantitative analysis of proteins secreted by Leishmania (Viannia) braziliensis strains associated to distinct clinical manifestations of American Tegumentary Leishmaniasis. Journal of Proteomics, v. 232, 104077, p. 1-15, 2021. | pt_BR |
| ISSN | 1874-3919 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46281 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Espectrometria de massa | pt_BR |
| Subject in Portuguese | Leishmaniose cutânea localizada | pt_BR |
| Subject in Portuguese | Leishmaniose cutânea disseminada | pt_BR |
| Subject in Portuguese | Proteômica quantitativa | pt_BR |
| Subject in Portuguese | Espectrometria de massa | pt_BR |
| Title | Quantitative analysis of proteins secreted by Leishmania (Viannia) braziliensis strains associated to distinct clinical manifestations of American Tegumentary Leishmaniasis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jprot.2020.104077 | |
| Abstract | The role of Leishmania braziliensis in the development of different clinical forms of American Tegumentary Leishmaniasis (ATL) is unclear, but it has been suggested that molecules secreted/released by parasites could modulate the clinical outcome. Here, we analyzed the infection rate and cytokine profile of macrophages pretreated with the secretome of two L. braziliensis strains associated with polar clinical forms of ATL: one associated with localized self-healing cutaneous leishmaniasis (LCL) and other associated with the disseminated form (DL). Besides, we use an iTRAQ-based quantitative proteomics approach to compare the abundance of proteins secreted by those strains. In vitro infection demonstrated that pretreatment with secretome resulted in higher number of infected macrophages, as well as higher number of amastigotes per cell. Additionally, macrophages pretreated with LCL secretome exhibited a proinflammatory profile, whereas those pretreated with the DL one did not. These findings suggest that secretomes made macrophages more susceptible to infection and that molecules secreted by each strain modulate, differentially, the macrophages’ cytokine profile. Indeed, proteomics analysis showed that the DL secretome is rich in molecules involved in macrophage deactivation, while is poor in proteins that activate proinflammatory pathways. Together, our results reveal new molecules that may contribute to the infection, persistence and dissemination of the parasite. Significance: Leishmania braziliensis is associated to localized self-healing cutaneous lesions (LCL), disseminated leishmaniasis (DL), and mucocutaneous lesions (MCL). To understand the role of the parasite in those distinct clinical manifestations we evaluated infection rates and cytokine profiles of macrophages pre-treated with secretomes of two L. braziliensis strains associated with DL and LCL, and quantitatively compared these secretomes. The infection index of macrophages pretreated with the DL secretome was significantly higher than that exhibited by non-treated cells. Interestingly, whereas the LCL secretome stimulated a proinflammatory setting, | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Antonio Nariño. Grupo de Investigación en Biología Celular y Funcional e Ingeniería de Biomoléculas. Bogotá, Colombia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, GB, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil / Universidade Federal de São João Del Rei, São João del Rei. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, GB, Brasil. | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Secretome | pt_BR |
| Subject | Localized cutaneous leishmaniasis | pt_BR |
| Subject | Disseminated cutaneous leishmaniasis | pt_BR |
| Subject | Quantitative proteomics | pt_BR |
| Subject | Mass spectrometry | pt_BR |
| Embargo date | 2025 | pt_BR |
