| Author | Sales, Caroline Brandi Schlaepfer | |
| Author | Guimarães, Vanessa S. N. | |
| Author | Valverde, Ludmila de Faro | |
| Author | Dias, Rosane Borges | |
| Author | Freitas, Raíza D. | |
| Author | Rocha, Leonardo de Oliveira Siquara da | |
| Author | Miranda, Malu Coelho de | |
| Author | Bôas, Deise S. Vilas | |
| Author | Agra, Ivan M. Gonçalves | |
| Author | Santos, Jean N. dos | |
| Author | Rocha, Clarissa Araújo Gurgel | |
| Access date | 2021-03-23T13:12:37Z | |
| Available date | 2021-03-23T13:12:37Z | |
| Document date | 2021 | |
| Citation | SALES, Caroline B. Schlaepfer et al. Glypican-1, -3, -5 (GPC1, GPC3, GPC5) and Hedgehog Pathway Expression in Oral Squamous Cell Carcinoma. Applied Immunohistochemistry & Molecular Morphology, 2021. | pt_BR |
| ISSN | 1541-2016 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46404 | |
| Language | eng | pt_BR |
| Publisher | Society for Applied Immunohistochemistry | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Neoplasias bucais | pt_BR |
| Subject in Portuguese | Reação em cadeia da polimerase em tempo real, | pt_BR |
| Subject in Portuguese | Imunohistoquímica | pt_BR |
| Subject in Portuguese | Proteínas | pt_BR |
| Subject in Portuguese | Proteoglicanas de Heparan Sulfato | pt_BR |
| Title | Glypican-1, -3, -5 (GPC1, GPC3, GPC5) and Hedgehog Pathway Expression in Oral Squamous Cell Carcinoma | pt_BR |
| Type | Article | pt_BR |
| Abstract | Proteoglycans are involved in tumor development and may
regulate the Hedgehog (HH) pathway. This study aimed to investigate
the gene and protein expression of glypican-1 (GPC1), -3 (GPC3), and
-5 (GPC5) in oral squamous cell carcinoma (OSCC) and tumor-free
lateral margins (TM) and their association with the HH pathway.
Quantitative PCR was performed for GPC1, GPC3, GPC5, SHH,
PTCH1, SMO, and GLI1 genes in samples of OSCC (n=31), TM
(n=12), and non-neoplastic oral mucosa (NNM) of healthy patients
(n=6), alongside an immunohistochemical evaluation of GPC1,
GPC3, and GPC5 proteins and HH proteins SHH and gliomaassociated
oncogene homolog 1 (GLI1). Double staining for GPC3/
SHH, GPC5/SHH, GPC3/tubulin [ac Lys40], GPC5/Tubulin [ac
Lys40], and GPC5/GLI1 was also performed. Overexpression of
GPC1 and GPC5 in tumor samples and underexpressed levels of
GPC3 gene transcripts were observed when compared with TM
(standard sample). HH pathway mRNA aberrant expression in
OSCC samples and a negative correlation between GPC1 and GPC5
at transcription levels were detected. GPC1 staining was rare in
OSCC, but positive cells were found in NNM and TM. Otherwise
positive immunostaining for GPC3 and GPC5 was observed in
OSCCs, but not in NNM and TM. Blood vessels adjacent to tumor
islands were positive for GPC1 and GPC5. Co-localization of
GPC3-positive and GPC5-positive cells with SHH and Tubulin [ac
Lys40] proteins was noted, as well as of GPC5 and GLI1. The absence
of the GPC1 protein in neoplastic cells, underexpression of the GPC3
gene, and co-localization of GPCs and HH proteins may indicate the
maintenance of aberrant HH pathway activation in OSCC. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Bahia. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Subject | Oral cancer | pt_BR |
| Subject | Real-time polymerase chain reaction | pt_BR |
| Subject | Immunohistochemistry | pt_BR |
| Subject | Hedgehog proteins | pt_BR |
| Subject | Heparan Sulfate proteoglycans | pt_BR |
