| Author | Rahman, Rafi Ur | |
| Author | Souza, Barbara | |
| Author | Uddin, Iftikhar | |
| Author | Carrara, Luana | |
| Author | Brito, Luiz Paulo | |
| Author | Costa, Monique Melo | |
| Author | Mahmood, Muhammad Asif | |
| Author | Khan, Sozaina | |
| Author | Lima, José Bento Pereira | |
| Author | Martins, Ademir Jesus | |
| Access date | 2021-04-06T19:39:46Z | |
| Available date | 2021-04-06T19:39:46Z | |
| Document date | 2021 | |
| Citation | RAHMAN, Rafi Ur et al. Insecticide resistance and underlying targets‑site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan. Scientifc Reports, v. 11, p. 1-15, 2021. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46526 | |
| Language | eng | pt_BR |
| Publisher | Nature | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Aedes aegypti | pt_BR |
| Subject in Portuguese | Aedes albopictus | pt_BR |
| Subject in Portuguese | Resistência a Inseticidas | pt_BR |
| Subject in Portuguese | Lahore, Pakistan | pt_BR |
| Subject in Portuguese | Mecanismos metabólicos | pt_BR |
| Title | Insecticide resistance and underlying targets‑site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-021-83465-w | |
| Abstract | Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat
to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus
populations from Lahore to WHO-recommended insecticides and to investigate metabolic and
target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides
temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alphacypermethrin,
and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR,
High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes
in resistance, we carried out synergist bioassay with both species and then checked the expression
of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species
were susceptible to organophosphates and the insect growth regulator, however resistant to all
pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high
frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased
the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring
resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city
are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450
overexpression. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Community Medicine Department, Bacha Khan Medical College Mardan. Mardan, Pakistan. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institute of Public Health. Lahore, Pakistan.. | pt_BR |
| Affilliation | Zoology Department, Government College University. Lahore, Pakistan. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. INCT-EM, UFRJ, Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Insecticide resistance | pt_BR |
| Subject | Aedes aegypti | pt_BR |
| Subject | Aedes albopictus | pt_BR |
| Subject | Lahore, Pakistan | pt_BR |
| Subject | Metabolic mechanisms | pt_BR |
