| Author | Solcà, Manuela da Silva | |
| Author | Arruda, Maiara Reis | |
| Author | Leite, Bruna Martins Macedo | |
| Author | Mota, Tiago Feitosa | |
| Author | Rebouças, Miriam Flores | |
| Author | Jesus, Matheus Silva de | |
| Author | Amorim, Leila Denise Alves Ferreira | |
| Author | Borges, Valéria Matos | |
| Author | Valenzuela, Jesus | |
| Author | Kamhawi, Shaden | |
| Author | Veras, Patrícia Sampaio Tavares | |
| Author | Fraga, Deborah Bittencourt Mothé | |
| Author | Brodskyn, Claudia Ida | |
| Access date | 2021-04-12T11:44:03Z | |
| Available date | 2021-04-12T11:44:03Z | |
| Document date | 2021 | |
| Citation | SOLCA, Manuela da Silva et al. Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort. PLOS Neglected Tropical Diseases, fev. 2021. | pt_BR |
| ISSN | 1935-2735 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46629 | |
| Sponsorship | Fundac¸ão de Amparo à Pesquisa do Estado da
Bahia-FAPESB (N˚SUS0036/2013 and PET0024/
2013 to C.I.B.); a grant from the Conselho Nacional
de Desenvolvimento Cientı´fico e Tecnolo´gico
(402670/2012-4 to C.I.B. and 400898/2013-6 to P.
S.T.V.); grants from the Instituto Nacional de Ciência e Tecnologia em Doenc¸as Tropicais (INCTDT)
(to D. B. M. F. and P. S. T. V.), from the
Intramural Research Programs at the NIAID, NIH,
USA (S.K., J.V.) and the Instituto Nacional de
Ciência e Tecnologia de Investigac¸ão em
Imunologia (III-INCT) (to C. I. B.). PrInt Fiocruz-
Capes (grant n˚01/2021) to C.I.B. and D.B.M.F. The
funders had no role in study design, data collection
and analysis, decision to publish, or preparation of
the manuscript. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Leishmaniose visceral | pt_BR |
| Subject in Portuguese | Saliva | pt_BR |
| Subject in Portuguese | Psychodidae | pt_BR |
| Subject in Portuguese | Cães | pt_BR |
| Subject in Portuguese | Infecções | pt_BR |
| Title | Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0009137 | |
| Abstract | Reports have shown correlations between the immune response to vector saliva and Leishmaniasis
outcome. We followed dogs in an endemic area for two years characterizing resistance
or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania
infantum diagnosis and clinical development criteria. Then, we aimed to identify a biosignature
based on parasite load, serum biological mediators’ interactions, and vector exposure
intensity associated with CVL resistance and susceptibility.
Methodology/Principal findings
A prospective two-year study was conducted in an area endemic for CVL. Dogs were evaluated
at 6-month intervals to determine infection, clinical manifestations, immune profile, and
sandfly exposure. CVL resistance or susceptibility was determined upon the conclusion of
the study. After two years, 78% of the dogs were infected with L. infantum (53% susceptible
and 47% resistant to CVL). Susceptible dogs presented higher splenic parasite load as well
as persistence of the parasite during the follow-up, compared to resistant ones. Susceptible
dogs also displayed a higher number of correlations among the investigated biological mediators,
before and after infection diagnosis. At baseline, anti-saliva antibodies, indicative of
exposure to the vector, were detected in 62% of the dogs, reaching 100% in one year.
Higher sandfly exposure increased the risk of susceptibility to CVL by 1.6 times (CI: 1.11–2.41). We identified a discriminatory biosignature between the resistant and susceptible
dogs assessing splenic parasite load, interaction of biological mediators, PGE2 serum levels
and intensity of exposure to sandfly. All these parameters were elevated in susceptible dogs
compared to resistant animals.
Conclusions/Significance
The biosignature identified in our study reinforces the idea that CVL is a complex multifactorial
disease that is affected by a set of factors which are correlated and, for a better understanding
of CVL, should not be evaluated in an isolated way. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Matemática e Estatística. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research,Rockville. Maryland, United States of America. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz, Instituto de Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia. Investigação em Imunologia. São Paulo, SP, Brasil. | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| Subject | Saliva | pt_BR |
| Subject | Psychodidae | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Infection | pt_BR |
