| Author | Mondêgo-Oliveira, Renata | |
| Author | Souza, Joyce Cortez de Sá | |
| Author | Moragas-Tellis, Carla Junqueira | |
| Author | Souza, Paulo Victor Ramos de | |
| Author | Chagas, Maria do Socorro dos Santos | |
| Author | Behrens, Maria Dutra | |
| Author | Hardoim, Daiana de Jesus | |
| Author | Taniwaki, Noemi Nosomi | |
| Author | Chometon, Thaize Quiroga | |
| Author | Berho, Alvaro Luiz | |
| Author | Calabrese, Kátia da Silva | |
| Author | Souza, Fernando Almeida | |
| Author | Silva, Ana Lucia Abreu | |
| Access date | 2021-04-16T18:32:23Z | |
| Available date | 2021-04-16T18:32:23Z | |
| Document date | 2021 | |
| Citation | MONDÊGO-OLIVEIRA, Renata et al. Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes. Biomedicine & Pharmacology, v. 133, 111025, p. 1-12, 2021. | pt_BR |
| ISSN | 0753-3322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46741 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | β-caryophyllene | pt_BR |
| Subject in Portuguese | Células DH82 | pt_BR |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Subject in Portuguese | Isobolograma | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Microscopia eletrônica de transmissão | pt_BR |
| Title | Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.biopha.2020.111025 | |
| Abstract | The present study aimed to evaluate the antileishmanial effect, the mechanisms of action and the association with miltefosine of Vernonia brasiliana essential oil against Leishmania infantum promastigotes. This essential oil was obtained by hydrodistillation and its chemical composition was determined by gas chromatography-mass spectrometry (GC–MS). The antileishmanial activity against L. infantum promastigotes and cytotoxicity on DH82 cells were evaluated by MTT colorimetric assay. Ultrastructural alterations were evaluated by transmission electron microscopy. Changes in mitochondrial membrane potential, in the production of reactive oxygen spe cies, and analysis of apoptotic events were determined by flow cytometry. The association between the essential oil and miltefosine was evaluated using the modified isobologram method. The most abundant component of the essential oil was β-caryophyllene (21.47 %). Anti-Leishmania assays indicated an IC50 of 39.01 ± 1.080 μg/mL for promastigote forms after 72 h of treatment. The cytotoxic concentration for DH82 cells was 63.13 ± 1.211 μg/mL after 24 h of treatment. The effect against L. infantum was proven through the ultrastructural changes caused by the oil, such as kinetoplast and mitochondrial swelling, vesicles in the flagellar pocket, discontinuity of the nuclear membrane, nuclear fragmentation and condensation, and loss of organelles. It was observed that the oil leads to a decrease in the mitochondrial membrane potential (35.10 %, p = 0.0031), increased reactive oxygen species production, and cell death by late apoptosis (17.60 %, p = 0.020). The combination of the essential oil and miltefosine exhibited an antagonistic effect. This study evidences the antileishmanial action of V. brasiliana essential oil against L. infantum promastigotes. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Universidade CEUMA. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Tecnologia em Fármacos. Programa de Pós-Graduação Acadêmico em Pesquisa Translacional em Fármacos e Medicamentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Adolfo Lutz. Núcleo de Microscopia Eletrônica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma Tecnológica de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhao. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. São Luís, MA, Brasil. | pt_BR |
| Subject | β-caryophyllene | pt_BR |
| Subject | DH82 cells | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Isobologram | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Transmission electron microscopy | pt_BR |
