| Author | Araujo, Anna Erika Vieira de | |
| Author | Conde, Luis Vidal | |
| Author | Silva Junior, Haroldo Cid da | |
| Author | Machado, Lucas de Almeida | |
| Author | Lara, Flavio Alves | |
| Author | Chapeaurouge, Alex | |
| Author | Pauer, Heidi | |
| Author | Hardoim, Cristiane Cassiolato Pires | |
| Author | Antunes, Luis Caetano Martha | |
| Author | Carvalho-Assef, Ana Paula D’Alincourt | |
| Author | Senna, José Procópio Moreno | |
| Access date | 2021-04-20T23:52:14Z | |
| Available date | 2021-04-20T23:52:14Z | |
| Document date | 2021 | |
| Citation | ARAUJO, Anna Erika Vieira de et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes, 104801, p. 1-34, Feb. 2021. | |
| ISSN | 1769-714X | |
| URI | https://www.arca.fiocruz.br/handle/icict/46794 | |
| Language | eng | en_US |
| Publisher | Elsevier Masson SAS | |
| Rights | open access | |
| Subject in Portuguese | Acinetobacter baumannii | pt_BR |
| Subject in Portuguese | BamA | pt_BR |
| Subject in Portuguese | iImmunogênico | pt_BR |
| Subject in Portuguese | Especificidade | pt_BR |
| Subject in Portuguese | Bactérias resistentes | pt_BR |
| Title | Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii | en_US |
| Type | Preprint | |
| DOI | 10.1016/j.micinf.2021.104801 | |
| Abstract | Acinetobacter baumannii is an important nosocomial pathogen. BamA is a protein that belongs to a complex responsible for organizing the proteins on the bacterial outer membrane. In this work, we aimed to evaluate murine immune responses to BamA recombinant protein (rAbBamA) from A. baumannii in an animal model of infection, and to assess cross-reactivity of this target for the development of anti-A. baumannii vaccines or diagnostics. Immunization of mice with rAbBamA elicited high antibody titers and antibody recognition of native A. baumannii BamA. Immunofluorescence also detected binding to the bacterial surface. After challenge, immunized mice demonstrated a 40% survival increase and better bacterial clearance in kidneys. Immunoblot of anti-rAbBamA against other medically relevant bacteria showed binding to proteins of approximately 35 kDa in Klebsiella pneumoniae and Escherichia coli lysates, primarily identified as OmpA and OmpC, respectively. Altogether, our data show that anti-rAbBamA antibodies provide a protective response against A. baumannii infection in mice. However, the response elicited by immunization with rAbBamA is not completely specific to A. baumannii. Although a broad-spectrum vaccine that protects against various pathogens is an appealing strategy, antibody reactivity against the human microbiota is undesired. In fact, immunization with rAbBamA produced noticeable effects on the gut microbiota. However, the changes elicited were small and non-specific, given that no significant changes in the abundance of Proteobacteria were observed. Overall, rAbBamA is a promising target, but specificity must be considered in the development of immunological tools against A. baumannii. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia da Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade de São Paulo. Instituto de Biociências. Coastal Campus de São Vicente, SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia da Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Subject | Acinetobacter baumannii | en_US |
| Subject | BamA | en_US |
| Subject | iImmunogenic | en_US |
| Subject | Specificity | en_US |
| Subject | Resistant bacteria | en_US |
