| Author | Araújo, Ana Carolina Justino de | pt_BR |
| Author | Freitas, Priscilla Ramos | pt_BR |
| Author | Barbosa, Cristina Rodrigues dos Santos | pt_BR |
| Author | Muniz, Débora Feitosa | pt_BR |
| Author | Ribeiro Filho, Jaime | pt_BR |
| Author | Tintino, Saulo Relison | pt_BR |
| Author | Siqueira Júnior, José Pinto de | pt_BR |
| Author | Barbosa Filho, José Maria | pt_BR |
| Author | Sousa, Gabriela Ribeiro de | pt_BR |
| Author | Coutinho, Henrique Douglas Melo | pt_BR |
| Access date | 2021-05-20T11:36:37Z | |
| Available date | 2021-05-20T11:36:37Z | |
| Document date | 2021 | |
| Citation | ARAÚJO, Ana Carolina Justino de et al. Modulation of drug resistance by limonene: inhibition of efflux pumps in Staphylococcus aureus strains RN-4220 and IS-58. Current Drug Metabolism, v. 22, n. 2, p. 110-113, 23 Mar. 2021. | pt_BR |
| ISSN | 1389-2002 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47253 | |
| Language | eng | en |
| Publisher | Bentham Science Publishers | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Staphylococcus aureus | pt_BR |
| Subject in Portuguese | Limoneno | pt_BR |
| Subject in Portuguese | Resistência a medicamnetos | pt_BR |
| Subject in Portuguese | Bombas | pt_BR |
| Subject in Portuguese | Etídio | pt_BR |
| Subject in Portuguese | Antibacterianos | pt_BR |
| Title | Modulation of drug resistance by limonene: inhibition of efflux pumps in Staphylococcus aureus strains RN-4220 and IS-58 | en |
| Type | Article | pt_BR |
| DOI | 10.2174/1389200221999210104204718 | pt_BR |
| Abstract | AIMS: This study aimed to investigate the potential of limonene as an efflux pump (EP) inhibitor in Staphylococcus aureus strains, RN-4220 and IS-58, which carry EPs for erythromycin (MrsA) and tetracycline (TetK), respectively. BACKGROUND: The evolution of bacterial resistance mechanisms over time has impaired the action of most classes of antibiotics. Staphylococcus aureus is a notable bacterium, with high pathogenic potential and demonstrated resistance to conventional antibiotics. Considering the importance of discovering novel compounds to combat antibiotic resistance, our group previously demonstrated the antibacterial properties of limonene, a compound present in the essential oils of several plant species. OBJECTIVE: This study aimed to investigate the potential of limonene as an efflux pump (EP) inhibitor in Staphylococcus aureus strains RN-4220 and IS-58, which carry EPs for erythromycin (MrsA) and tetracycline (TetK), respectively. METHODS: The minimum inhibitory concentrations (MIC) of limonene and other efflux pump inhibitors were determined through the broth microdilution method. A reduction in the MIC of ethidium bromide was used as a parameter of EP inhibition. RESULT: While limonene was not shown to exhibit direct antibacterial effects against EP-carrying strains, in association with ethidium bromide and antibiotics, this compound demonstrated enhanced antibacterial activity, indicating the inhibition of the MrsA and TetK pumps. CONCLUSION: In conclusion, this pioneering study demonstrated the effectiveness of limonene as an EP inhibitor in S. aureus strains, RN-4220 and IS-58. Nevertheless, further studies are required to characterize the molecular mechanisms associated with limonene-mediated EP inhibition. | en |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Regional do Cariri. Centro de Ciências Biológicas e da Saúde. Departamento de Química Biológica. Crato, CE, Brasil. | pt_BR |
| Subject | Staphylococcus aureus | en |
| Subject | Limonene | en |
| Subject | Bacterial resistance | en |
| Subject | Efflux pump inhibitors | en |
| Subject | Ethidium bromide | en |
| Subject | Antibacterial | en |
