Author | Jácome, Fernanda Cunha | |
Author | Caldas, Gabriela Cardoso | |
Author | Rasinhas, Arthur da Costa | |
Author | Almeida, Ana LuisaTeixeira de | |
Author | Souza, Daniel Dias Coutinho de | |
Author | Paulino, Amanda Carlos | |
Author | Leonardo, Raphael | |
Author | Barth, Ortrud Monika | |
Author | Santos, Flavia Barreto dos | |
Author | Vieira, Débora Ferreira Barreto | |
Access date | 2021-05-25T19:07:31Z | |
Available date | 2021-05-25T19:07:31Z | |
Document date | 2021 | |
Citation | JÁCOME, Fernanda Cunha et al. Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model. Scientific Reports, v. 11, n. 9723, 18p, 2021. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/47366 | |
Language | eng | pt_BR |
Publisher | Nature | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Linhagens DENV-2 | pt_BR |
Subject in Portuguese | Fígado | pt_BR |
Subject in Portuguese | Modelo murino imunocompetente | pt_BR |
Subject in Portuguese | Análise comparativa | pt_BR |
Title | Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-021-88502-2 | |
Abstract | Dengue (DEN) is the most prevalent arbovirus among humans, and four billion people live at risk of
infection. The clinical manifestations of DEN are variable, and the disease may present subclinically
or asymptomatically. A quarter of patients develop classical dengue (CD) or severe dengue (SD),
which is potentially lethal and involves vascular permeability changes, severe hemorrhage and
organ damage. The involvement of the liver is a fairly common feature in DEN, and alterations range
from asymptomatic elevation of transaminases to acute liver failure. Since its introduction in Brazil
in 1990, two strains of Dengue virus (DENV) serotype 2 (DENV-2) have been detected: Lineage I,
which is responsible for an outbreak in 1991, and Lineage II, which caused an epidemic greater than
the previous one and had a diferent epidemiological profle. To date, studies on diferent strains of
the same serotype/genotype and their association with disease severity are scarce. In addition, one
of the greatest challenges regarding the study of DEN pathogenesis and the development of drug
and vaccine therapies is the absence of an animal model that reproduces the disease as it occurs in
humans. The main goals of this study were to assess BALB/c mouse susceptibility experimentally
infected by two distinct DENV-2 strains and characterize possible diferences in the clinical signs and
alterations induced in the liver resulting from those infections. Mice infected by the two DENV-2
lineages gained less weight than uninfected mice; however, their livers were slightly heavier. Increased
AST and AST levels were observed in infected mice, and the number of platelets increased in the frst
72 h of infection and subsequently decreased. Mice infected with both lineages presented leukocytosis
but at diferent times of infection. The histopathological changes induced by both lineages were
similar and comparable to the changes observed in DEN fatal cases. The viral genome was detected in
two liver samples. The results demonstrate the susceptibility of BALB/c mice to both DENV-2 lineages
and suggest that the changes induced by those strains are similar, although for some parameters, they
are manifested at diferent times of infection. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Comparative analysis | pt_BR |
Subject | Liver disease | pt_BR |
Subject | Modelo murino imunocompetente | pt_BR |
Subject | DENV‑2 lineages | pt_BR |