| Author | Ramos, Aline de Souza | |
| Author | Ribeiro, Joyce Benzaquem | |
| Author | Teixeira, Bruna Gomes | |
| Author | Ferreira, José Luiz Pinto | |
| Author | Silva, Jefferson Rocha de Andrare | |
| Author | Ferreira, Alexandre do Amaral | |
| Author | Souza, Rodrigo Octavio Mendonça Alves de | |
| Author | Amaral, Ana Claudia Fernandes | |
| Access date | 2021-06-01T12:16:45Z | |
| Available date | 2021-06-01T12:16:45Z | |
| Document date | 2015 | |
| Citation | RAMOS, Aline de Souza et al. Hydroxylation of 1,8-cineole by Mucor ramannianus and Aspergillus niger. Brazilian Journal of Microbiology, v. 46, n. 1, p. 261-264, Jan./Mar. 2015. | pt_BR |
| ISSN | 1678-4405 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47471 | |
| Language | por | pt_BR |
| Publisher | Sociedade Brasileira de Microbiologia | |
| Rights | open access | pt_BR |
| Title | Hydroxylation of 1,8-cineole by Mucor ramannianusand Aspergillus niger | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/S1517-838246120131020 | |
| Abstract | The monoterpenoid 1,8-cineole is obtained from the leaves of Eucalyptus globulus and it has important biological activities. It is a cheap natural substrate because it is a by-product of the Eucalyptuscultivation for wood and pulp production. In this study, it was evaluated the potential of three filamentous fungi in the biotransformation of 1,8-cineole. The study was divided in two steps: first, reactions were carried out with 1,8-cineole at 1 g/L for 24 h; afterwards, reactions were carried out with substrate at 5 g/L for 5 days. The substrate was hydroxylated into 2-exo-hydroxy-1,8-cineole and 3-exo-hydroxy-1,8-cineole by fungi Mucor ramannianus and Aspergillus niger with high stereoselectivity. Trichoderma harzianum was also tested but no transformation was detected. M. ramannianus led to higher than 99% of conversion within 24 h with a starting high substrate concentration (1 g/L). When substrate was added at 5 g/L, only M. ramannianuswas able to catalyze the reaction, but the conversion level was 21.7% after 5 days. Both products have defined stereochemistry and could be used as chiral synthons. Furthermore, biological activity has been described for 3-exo-hydroxy-1,8-cineol. To the best of our knowledge, this is the first report on the use of M. ramannianus in this reaction. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Faculdade de Farmácia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Biotransformation | pt_BR |
| Subject | Monoterpene | pt_BR |
| Subject | Eucalyptol | pt_BR |
| Subject | Mucor Ramannianus | pt_BR |
| Subject | Aspergillus Niger | pt_BR |
