| Author | Medeiros, Fernanda Alvarenga Cardoso | |
| Author | Souza Filho, Job Alves de | |
| Author | Barbosa, José Ronaldo | |
| Author | Donato, Lucas Edel | |
| Author | Figueiredo, Fabiano Borges | |
| Author | Werneck, Guilherme Loureiro | |
| Author | Paz, Gustavo Fontes | |
| Author | Thompson, Mayerson | |
| Author | Marcelino, Andreza Pain | |
| Access date | 2021-06-14T20:42:43Z | |
| Available date | 2021-06-14T20:42:43Z | |
| Document date | 2021 | |
| Citation | MEDEIROS, Fernanda Alvarenga Cardoso et al. Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil. Cadernos de Saúde Pública, Rio de Janeiro, v. 37, n. 3, p. 1–11, 2021. | pt_BR |
| ISSN | 1678-4464 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47682 | |
| Language | por | pt_BR |
| Publisher | Fiocruz/ENSP | pt_BR |
| Rights | open access | pt_BR |
| Title | Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0102-311X00041320 | |
| Abstract | Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISAVetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Ministério da Saúde. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Enzyme-Linked Immunosorbent Assay | pt_BR |
| Subject | Leishmaniasis, Visceral | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject in Spanish | Ensayo de Inmunoadsorción Enzimática | pt_BR |
| Subject in Spanish | Leishmaniasis Visceral | pt_BR |
| Subject in Spanish | Perros | pt_BR |
| Subject in French | Test ELISA | pt_BR |
| Subject in French | Leishmaniose viscérale | pt_BR |
| Subject in French | Chiens | pt_BR |
| Subject in French | Diagnostic | pt_BR |
| DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
| DeCS | Leishmaniose Visceral | pt_BR |
| DeCS | Cães | pt_BR |
| DeCS | Diagnóstico | pt_BR |
