| Author | Kovach, Jeffrey D. | pt_BR |
| Author | Long, Jeffrey C. | pt_BR |
| Author | Barbosa, Lucio M. | pt_BR |
| Author | Moura, Ana Rafaela Silva Simões | pt_BR |
| Author | Silva, Luciano Kalabric | pt_BR |
| Author | Reis, Mitermayer Galvão dos | pt_BR |
| Author | Blanton, Ronald Edward | pt_BR |
| Access date | 2021-06-22T16:55:18Z | |
| Available date | 2021-06-22T16:55:18Z | |
| Document date | 2021 | |
| Citation | KOVACH, Jeffrey D. et al. A Schistosoma mansoni tri- and tetramer microsatellite catalog for genetic population diversity and differentiation. International Journal for Parasitology, p. 1-8, 19 May 2021. | pt_BR |
| ISSN | 0020-7519 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47840 | |
| Sponsorship | National Institutes of Health, USA Grant R01AI121330 | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Schistosoma mansoni | pt_BR |
| Subject in Portuguese | Genética | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Quênia | pt_BR |
| Subject in Portuguese | Infecções | pt_BR |
| Subject in Portuguese | População | pt_BR |
| Title | A Schistosoma mansoni tri- and tetramer microsatellite catalog for genetic population diversity and differentiation | en_US |
| Type | Article | |
| DOI | 10.1016/j.ijpara.2021.04.002 | pt_BR |
| Abstract | All Schistosoma mansoni tri- and tetranucleotide repeat microsatellites published as of December 2018 were identified. All 52 were evaluated for autosomal location, strength of amplification, scorability and behavior as single-copy loci by polyacrylamide and capillary gel electrophoresis. Of these, 27 were unique, autosomal, polymorphic, easily scored and single copy as assessed on pooled adult worm DNA from two different continental origins and adult worm clones. These microsatellites were distributed across all seven autosomal chromosomes. On laboratory strains their heterozygosity ranged from 0.22 to 0.77. Individual markers had 5–13 alleles, allelic richness of 2–10 and an effective allele number of 1.3–8.14. Those infected by Schistosoma mansoni carry many genetically distinct, sexually reproducing parasites, therefore, for an individual infection the complete allele frequency profile of their progeny consists of a pool of DNA from multiple diploid eggs. Using a set of 25 microsatellites, we calculated allele frequency profiles of eggs in fecal samples from people in two Brazilian communities separated by 6 km: Jenipapo (n = 80) and Volta do Rio (n = 38). There were no a priori characteristics that could predict the performance of markers in natural infections based on their performance with laboratory strains. Increasing marker number did not change accuracy for differentiation and diversity but did improve precision. Our data suggest that using a random set of 10–20 microsatellites appears to result in values that exhibit low standard deviations for diversity and differentiation indices. All identified microsatellites as well as PCR conditions, allele size, primer sequences and references for all tri- and tetramer microsatellites markers presented in this work are available at: https://sites.google.com/case.edu/cwru-and-fiocruz-wdrc/home. | en |
| Affilliation | Case Western Reserve University. Center for Global Health and Diseases. Biomedical Research Building. Cleveland, OH, USA. | pt_BR |
| Affilliation | University of New Mexico. Department of Anthropology. Albuquerque, NM, USA. | pt_BR |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Yale University. Yale School of Public Health. New Haven, CT, USA. | pt_BR |
| Affilliation | Tulane School of Public Health and Tropical Medicine. Department of Tropical Medicine. New Orleans, LA, USA. | pt_BR |
| Subject | Population genetics | en |
| Subject | Differentiation | en |
| Subject | Brazil | en |
| Subject | Kenya | en |
| Subject | Pooled samples | pt_BR |
