| Author | Chaves, Yuri Oliveira | |
| Author | Pereira, Flávio Ribeiro | |
| Author | Pinheiro, Rebeca de Souza | |
| Author | Batista, Diego Rafael Lima | |
| Author | Balieiro, Antônio Alcirley da Silva | |
| Author | Lacerda, Marcus Vinicius Guimarães de | |
| Author | Nogueira, Paulo Afonso | |
| Author | Guimarães, Monick Lindenmeyer | |
| Access date | 2021-06-24T18:51:11Z | |
| Available date | 2021-06-24T18:51:11Z | |
| Document date | 2021 | |
| Citation | CHAVES, Yuri Oliveira et al. High Detection Rate of HIV Drug Resistance Mutations among Patients Who Fail Combined Antiretroviral Therapy in Manaus, Brazil. BioMed Resarch International, v. 2021, Article ID 5567332, 9 p, 2021. | pt_BR |
| ISSN | 2314-6141 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47861 | |
| Language | eng | pt_BR |
| Publisher | Hindawi | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Alta taxa de detecção | pt_BR |
| Subject in Portuguese | Mutações de resistência a medicamentos | pt_BR |
| Subject in Portuguese | Pacientes com HIV | pt_BR |
| Subject in Portuguese | Falha na terapia antirretroviral combinada | pt_BR |
| Subject in Portuguese | Manaus, Brasil | pt_BR |
| Title | High Detection Rate of HIV Drug Resistance Mutations among Patients Who Fail Combined Antiretroviral Therapy in Manaus, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1155/2021/5567332 | |
| Abstract | Virologic failure may occur because of poor treatment adherence and/or viral drug resistance mutations (DRM). In Brazil, the
northern region exhibits the worst epidemiological scenarios for the human immunodeficiency virus (HIV). Thus, this study is
aimed at investigating the genetic diversity of HIV-1 and DRM in Manaus. The cross-sectional study included people living
with HIV on combined antiretroviral therapy and who had experienced virological failure during 2018-2019. Sequencing of the
protease/reverse transcriptase (PR/RT) and C2V3 of the viral envelope gp120 (Env) regions was analyzed to determine
subtypes/variants of HIV-1, DRMs, and tropism. Ninety-two individuals were analyzed in the study. Approximately 72% of
them were male and 74% self-declared as heterosexual. Phylogenetic inference (PR/RT-Env) showed that most sequences were B
subtype, followed by BF1 or BC mosaic genomes and few F1 and C sequences. Among the variants of subtype B at PR/RT,
84.3% were pandemic (BPAN), and 15.7% were Caribbean (BCAR). The DRMs most frequent were M184I/V (82.9%) for
nucleoside reverse transcriptase inhibitors (NRTI), K103N/S (63.4%) for nonnucleoside reverse transcriptase inhibitor (NNRTI),
and V82A/L/M (7.3%) for protease inhibitors (PI). DRM analysis depicted high levels of resistance for lamivudine and efavirenz
in over 82.9% of individuals; although, low (7.7%) cross-resistance to etravirine was observed. A low level of resistance to
protease inhibitors was found and included patients that take atazanavir/ritonavir (16.6%) and lopinavir (11.1%), which
confirms that these antiretrovirals can be used—for most individuals. The thymidine analog mutations-2 (TAM-2) resistance
pathway was higher in BCAR than in BPAN. Similar results from other Brazilian studies regarding HIV drug resistance were
observed; however, we underscore a need for additional studies regarding subtype BCAR variants. Molecular epidemiology
studies are an important tool for monitoring the prevalence of HIV drug resistance and can influence the public health policies. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manaus. AM, Brasil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manaus AM, Brasil. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manaus AM, Brasil. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manausm AM, Brasil. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manaus AM, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil.. | pt_BR |
| Affilliation | Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Diagnóstico e Controle de Doenças Infecciosas na Amazônia. Manaus AM, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Instituto de Pesquisa Clínica Carlos Borborema. Manaus, AM, Brasil.. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | High Detection Rate | pt_BR |
| Subject | Drug Resistance Mutations | pt_BR |
| Subject | Patients | pt_BR |
| Subject | Failure of treatment | pt_BR |
| Subject | Combined Antiretroviral Therapy | pt_BR |
| Subject | Manaus, Brazil | pt_BR |
| Subject | HIV | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
