| Author | Santos, Gabriel Menezes Costa dos | |
| Author | Alves, Carlos Roberto | |
| Author | Pinto, Marcelo Alves | |
| Author | Leon, Luciane Almeida Amado | |
| Author | Silva, Franklin Souza | |
| Access date | 2021-06-26T18:23:37Z | |
| Available date | 2021-06-26T18:23:37Z | |
| Document date | 2021 | |
| Citation | SANTOS, Gabriel Menezes Costa dos et al. Detection of Antibodies against Hepatitis A Virus (HAV) by a Surface Plasmon Resonance (SPR) Biosensor: A New Diagnosis Tool Based on the Major HAV Capsid Protein VP1 (SPR-HAVP1). Sensors, v. 21, n.3167, 11 p, May 2021. | pt_BR |
| ISSN | 1424-8220 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47882 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Vìrus da Hepatite A | pt_BR |
| Subject in Portuguese | IgM | pt_BR |
| Subject in Portuguese | Ressonância de Plasmônio de Superfície | pt_BR |
| Title | Detection of Antibodies against Hepatitis A Virus (HAV) by a Surface Plasmon Resonance (SPR) Biosensor: A New Diagnosis Tool Based on the Major HAV Capsid Protein VP1 (SPR-HAVP1) | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3390/s21093167 | |
| Abstract | Abstract: Hepatitis A (HA) is an acute human infectious disease caused by a positive single-stranded
RNA virus (HAV). It is mainly acquired through the fecal–oral route and is primarily spread by
contact between people and exposure to contaminated water and food. Recently, large outbreaks
of HA have been reported by low and moderate endemicity countries, emphasizing its importance
in public health and the need for rapid and large-scale diagnostic tests to support public health
decisions on HA. This work proposes a new tool for HAV diagnosis based on the association of
surface plasmonic resonance with major capsid protein VP1 (SPR-HAVP1 assay), detecting IgM
antibodies for HAV in human serum samples. Structural analyses of VP1 B-lymphocyte epitopes
showed continuous and discontinuous epitopes. The discontinuous epitopes were identified in
the N-terminal region of the VP1 protein. Both epitope types in the VP1 protein were shown by
the reactivity of VP1 in native and denaturing conditions to IgM anti-HAV, which was favorable
to tests of VP1 in the SPR assays. SPR-HAVP1 assays showed good performance in the detection
of IgM polyclonal antibody anti-HAV. These assays were performed using a COOH5 sensor chip
functionalized with VP1 protein. The sensorgram record showed a significant difference between
positive and negative serum samples, which was confirmed by analysis of variation of initial and
final dissociation values through time (∆RUd/t). The data gathered here are unequivocal evidence
that the SPR-HAVP1 strategy can be applied to detect IgM antibodies in human serum positive to the
HAV. This is a new tool to be explored to diagnose human HAV infections. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas (INCT/IDPN). Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Faculdade de Ciências Biológicas e da Saúde. Nova Iguaçu, RJ, Brasil. | pt_BR |
| Subject | Hepatitis A virus | pt_BR |
| Subject | Major capsid protein VP1 | pt_BR |
| Subject | IgM | pt_BR |
| Subject | Surface plasmon resonance | pt_BR |
| DeCS | Ressonância de Plasmônio de Superfície | pt_BR |
