Mostrar el registro sencillo del ítem

AutorSantos, Gabriel Menezes Costa dos
AutorAlves, Carlos Roberto
AutorPinto, Marcelo Alves
AutorLeon, Luciane Almeida Amado
AutorSilva, Franklin Souza
Fecha de acceso2021-06-26T18:23:37Z
Fecha de disponibilización2021-06-26T18:23:37Z
Fecha de publicación2021
ReferenciaSANTOS, Gabriel Menezes Costa dos et al. Detection of Antibodies against Hepatitis A Virus (HAV) by a Surface Plasmon Resonance (SPR) Biosensor: A New Diagnosis Tool Based on the Major HAV Capsid Protein VP1 (SPR-HAVP1). Sensors, v. 21, n.3167, 11 p, May 2021.pt_BR
ISSN1424-8220pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/47882
Idiomaengpt_BR
EditorMDPIpt_BR
Derechos de autoropen access
Palabras clave en PortuguésVìrus da Hepatite Apt_BR
Palabras clave en PortuguésIgMpt_BR
Palabras clave en PortuguésRessonância de Plasmônio de Superfíciept_BR
TítuloDetection of Antibodies against Hepatitis A Virus (HAV) by a Surface Plasmon Resonance (SPR) Biosensor: A New Diagnosis Tool Based on the Major HAV Capsid Protein VP1 (SPR-HAVP1)pt_BR
Tipo del documentoArticle
DOI10.3390/s21093167
Resumen en InglésAbstract: Hepatitis A (HA) is an acute human infectious disease caused by a positive single-stranded RNA virus (HAV). It is mainly acquired through the fecal–oral route and is primarily spread by contact between people and exposure to contaminated water and food. Recently, large outbreaks of HA have been reported by low and moderate endemicity countries, emphasizing its importance in public health and the need for rapid and large-scale diagnostic tests to support public health decisions on HA. This work proposes a new tool for HAV diagnosis based on the association of surface plasmonic resonance with major capsid protein VP1 (SPR-HAVP1 assay), detecting IgM antibodies for HAV in human serum samples. Structural analyses of VP1 B-lymphocyte epitopes showed continuous and discontinuous epitopes. The discontinuous epitopes were identified in the N-terminal region of the VP1 protein. Both epitope types in the VP1 protein were shown by the reactivity of VP1 in native and denaturing conditions to IgM anti-HAV, which was favorable to tests of VP1 in the SPR assays. SPR-HAVP1 assays showed good performance in the detection of IgM polyclonal antibody anti-HAV. These assays were performed using a COOH5 sensor chip functionalized with VP1 protein. The sensorgram record showed a significant difference between positive and negative serum samples, which was confirmed by analysis of variation of initial and final dissociation values through time (∆RUd/t). The data gathered here are unequivocal evidence that the SPR-HAVP1 strategy can be applied to detect IgM antibodies in human serum positive to the HAV. This is a new tool to be explored to diagnose human HAV infections.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas (INCT/IDPN). Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Faculdade de Ciências Biológicas e da Saúde. Nova Iguaçu, RJ, Brasil.pt_BR
Palavras clave en InglêsHepatitis A viruspt_BR
Palavras clave en InglêsMajor capsid protein VP1pt_BR
Palavras clave en InglêsIgMpt_BR
Palavras clave en InglêsSurface plasmon resonancept_BR
DeCSRessonância de Plasmônio de Superfíciept_BR


Ficheros en el ítem

application/pdf
Nombre:
GabrielSantos_marceloPinto_eta ...
Tamaño:
4.620Mb
Formato:
application/
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem