| Author | Assis, Gabriela Maira Pereira de | |
| Author | Alvarenga, Denise Anete Madureira de | |
| Author | Pereira, Matheus de Oliveira Costa | |
| Author | Sánchez-Arcila, Juan Camilo | |
| Author | Costa, Anielle de Pina | |
| Author | Souza Junior, Júlio César de Souza | |
| Author | Nunes, Ana Julia Dutra | |
| Author | Pissinatti, Alcides | |
| Author | Moreira, Silvia Bahadian | |
| Author | Torres, Leticia de Menezes | |
| Author | Costa, Helena Lott | |
| Author | Tinoco, Herlandes da Penha | |
| Author | Pereira, Valéria do Socorro | |
| Author | Soares, Irene da Silva | |
| Author | Souza, Taís Nóbrega de | |
| Author | Nutmngia, Francis Babila | |
| Author | Adams, John H. | |
| Author | Kano, Flora Satiko | |
| Author | Hirano, Zelinda Maria Braga | |
| Author | Daniel-Ribeiro, Claudio Tadeu | |
| Author | Ferreira, Joseli Oliveira | |
| Author | Carvalho, Luzia Helena | |
| Author | Brito, Cristiana Ferreira Alves de | |
| Access date | 2021-06-28T12:09:59Z | |
| Available date | 2021-06-28T12:09:59Z | |
| Document date | 2021 | |
| Citation | ASSIS, Gabriela Maria Pereira de et al. Profiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest. Front. Cell. Infect. Microbiol., v. 11, Article 678996, 15 p, May 2021. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/47889 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Malária | pt_BR |
| Subject in Portuguese | Imunidade Humoral | pt_BR |
| Subject in Portuguese | Primatas neotropicais | pt_BR |
| Subject in Portuguese | Mata Atlântica | pt_BR |
| Subject in Portuguese | Antígeno pré-eritrócitico | pt_BR |
| Subject in Portuguese | Antígenos eritróciticos | pt_BR |
| Title | Profiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest | pt_BR |
| Type | Article | |
| DOI | 10.3389/fcimb.2021.678996 | |
| Abstract | Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an
extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally
acquired humoral immune response against pre-erythrocytic and erythrocytic antigens
of Neotropical primates (NP) was evaluated here to improve the knowledge about the
exposure of those animals to the malaria transmission and support the identification of the
potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys
from three areas of the Atlantic Forest were used to identify IgG antibodies against
peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite
protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium
malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies
against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1
(PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding
protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity, Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an
extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally
acquired humoral immune response against pre-erythrocytic and erythrocytic antigens
of Neotropical primates (NP) was evaluated here to improve the knowledge about the
exposure of those animals to the malaria transmission and support the identification of the
potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys
from three areas of the Atlantic Forest were used to identify IgG antibodies against
peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite
protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium
malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies
against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1
(PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding
protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity,Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an
extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally
acquired humoral immune response against pre-erythrocytic and erythrocytic antigens
of Neotropical primates (NP) was evaluated here to improve the knowledge about the
exposure of those animals to the malaria transmission and support the identification of the
potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys
from three areas of the Atlantic Forest were used to identify IgG antibodies against
peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite
protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium
malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies
against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1
(PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding
protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity and captivity, potentially associated with humoral immune response were analyzed.
Eighty-five percent of NP had antibodies against at least one CSP peptide, and 76%
against at least one P. vivax erythrocytic antigen. A high percentage of adults compared to
non-adults were seropositive and showed increased antibody levels. Neotropical primates
with PCR positive for P. simium had a significantly higher frequency of positivity rate for
immune response against PvEBP-2, PvDBPII and also higher antibody levels against
PvDBPII, compared to PCR negative NPs for this species. Monkeys with PCR positive for
P. brasilianum/P. malariae showed higher frequency of seropositivity and antibody levels
against Pb/PmCSP. Levels of antibodies against Pb/PmCSP, PvEBP-2 and PvDBPII were
higher in free-living than in captive monkeys from the same area. All Platyrrhine families
showed antibodies against CSP peptides, however not all showed IgG against
erythrocytic antigens. These findings showed a high prevalence of naturally acquired
antibodies against CSP repeats in all studied areas, suggesting an intense exposure to
infected-mosquitoes bites of NP from all families. However, mainly monkeys of Atelidae
family showed antibodies against P. vivax erythrocytic antigens, suggesting blood
infection, which might serve as potential reservoirs of malaria in the Atlantic Forest. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Ambulatório de Doenças febris, Instituto Nacional de Infectologia (INI), Ambulatório de Doenças Febris Agudas, Rio de Janeiro, RJ, Brasil / Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis, RJ, Brasil. | pt_BR |
| Affilliation | Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brasil / Fundação Universidade Regional de Blumenau (FURB), Blumenau, SC, Brasil. | pt_BR |
| Affilliation | Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brasil / Programa de conservação do Bugio Ruivo. Joinville, SC, Brasil. | pt_BR |
| Affilliation | Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis, RJ, Brasil / Instituto Estadual do Ambiente (INEA). Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Estadual do Ambiente (INEA). Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação de Parques Municipais e Zoobotânica (FPMZB). Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação de Parques Municipais e Zoobotânica (FPMZB). Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, FL, USA. | pt_BR |
| Affilliation | Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, FL, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brasil / Fundação Universidade Regional de Blumenau (FURB), Blumenau, SC, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. Fundação Oswaldo Cruz. Instituto | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Malaria | pt_BR |
| Subject | Humoral response | pt_BR |
| Subject | Neotropical primates | pt_BR |
| Subject | Atlantic Forest | pt_BR |
| Subject | Pre-erythrocytic antigen | pt_BR |
| Subject | Erythrocytic antigens | pt_BR |
| DeCS | Imunidade Humoral | pt_BR |
| xmlui.metadata.dc.subject.ods | 15 Vida terrestre | |
