| Author | Araujo, Paula Finamore | |
| Author | Pereira, Amanda Faler | |
| Author | Brito, Carlos Ramon do Nascimento | |
| Author | Peres, Eldrinei Gomes | |
| Author | Yamaguchi, Klenicy Kazumy de Lima | |
| Author | Ferreira, Renata Trotta Barroso | |
| Author | Moreira, Octacilio Cruz | |
| Access date | 2021-07-15T13:38:54Z | |
| Available date | 2021-07-15T13:38:54Z | |
| Document date | 2021 | |
| Citation | ARAUJO, Paula Finamore et al. Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp. Plos One, v. 16, n. 2, e0246435, 17 p, Feb. 2021. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48247 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Validação | pt_BR |
| Subject in Portuguese | Novo ensaio PCR multiplex | pt_BR |
| Subject in Portuguese | Tempo real | pt_BR |
| Subject in Portuguese | Detecção | pt_BR |
| Subject in Portuguese | Quantificação | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Polpa de açai | pt_BR |
| Title | Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0246435 | |
| Abstract | In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mech anisms from public health perspective. Around 70% of new Chagas disease cases have
been associated with consumption of contaminated food or beverages. Ac¸ai (Euterpe olera cea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in
the Brazilian and international markets. Therefore, it has become important to incorporate in
the production process some procedures to measure out effective hygiene and product
quality control required by global market. Molecular methods have been developed for rapid
detection and quantification of T. cruzi DNA in several biological samples, including food
matrices, for epidemiological investigation of Chagas disease and food quality control. How ever, a high-performance molecular methodology since DNA extraction until detection and
quantification of T. cruzi DNA in ac¸ai berry pulp is still needed. Herein, a simple DNA extrac tion methodology was standardized from the supernatant of ac¸ai berry pulp stabilized in a
6M Guanidine-HCl/0.2M EDTA buffer. In addition, a multiplex real time qPCR assay, target ing T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated,
using reference from all T. cruzi DTUs and commercial samples of ac¸ai pulp, from an
endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in ac¸ai, was
reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This
high-sensitive, fast, and easy-to-use molecular assay is compatible with most of the labora tories involved in the investigations of oral Chagas disease outbreaks, representing an
important tool to the epidemiology, control, and surveillance of Chagas disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Anáises Clínicas e Toxicológicas. Natal, RN, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Departamento de Quimica. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Instituto de Saúde e Biotecnologia. Campus Coari, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Validation | pt_BR |
| Subject | Novel multiplex real-time PCR assay | pt_BR |
| Subject | Trypanosoma cruzi detection | pt_BR |
| Subject | Quantification | pt_BR |
| Subject | Açai pulp | pt_BR |
