Author | Pérez, Cindy | |
Author | Díaz-Roa, Andrea | |
Author | Bernal, Yuli | |
Author | Arenas, Nelson E. | |
Author | Kalume, Dario Eluan | |
Author | Côrtes, Luzia Monteiro de Castro | |
Author | Silva Junior, Pedro I. da | |
Author | Varela, Yahson | |
Author | Patarroyo, Manuel A. | |
Author | Torres, Orlando | |
Author | Bello, Felio J. | |
Access date | 2021-07-16T12:43:21Z | |
Available date | 2021-07-16T12:43:21Z | |
Document date | 2021 | |
Citation | PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/48253 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | |
Subject in Portuguese | Sarconesiopsis magellanica | pt_BR |
Subject in Portuguese | Peptídeos antimicrobianos | pt_BR |
Subject in Portuguese | Corpo de gordura larval | pt_BR |
Subject in Portuguese | Caracterização físico-química | pt_BR |
Subject in Portuguese | Avaliação antibacteriana | pt_BR |
Title | Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides | pt_BR |
Type | Article | |
DOI | 10.1590/0074-02760200587 | |
Abstract | BACKGROUND The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for
new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need.
OBJECTIVE Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs.
METHODS Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass
spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for
analysing the AMPs’ physico-chemical properties. Synthetic AMPs’ antibacterial activity was evaluated.
FINDINGS Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and
sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that
sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from
their native molecules’ sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative
and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect.
MAIN CONCLUSIONS S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could
be used in different antimicrobial therapies and overcoming bacterial resistance, | pt_BR |
Affilliation | Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia. | pt_BR |
Affilliation | Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil / Universidad Nacional Abierta y a Distancia, Escuela de Ciencias Agrícolas, Pecuarias y de Medio Ambiente. Bogotá, Colombia. | pt_BR |
Affilliation | Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia. | pt_BR |
Affilliation | Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia. | pt_BR |
Affilliation | Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia / Universidad Nacional de Colombia, Faculty of Medicine, Microbiology Department, Bogotá, Colombia / Universidad Santo Tomás, Health Sciences Division. Bogotá, Colombia. | pt_BR |
Affilliation | Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia. | pt_BR |
Affilliation | Universidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria. Bogotá, Colombia. | pt_BR |
Subject | Sarconesiopsis magellanica | pt_BR |
Subject | Antimicrobial peptides | pt_BR |
Subject | Larval fat body | pt_BR |
Subject | Physicochemical characterisation | pt_BR |
Subject | Antibacterial evaluation | pt_BR |