| Author | Calegar, Deiviane Aparecida | |
| Author | Monteiro, Kerla Joeline Lima | |
| Author | Bacelar, Polyanna Araújo Alves | |
| Author | Evangelista, Brenda Bulsara Costa | |
| Author | Almeida, Mayron Morais | |
| Author | Santos, Jéssica Pereira dos | |
| Author | Boia, Márcio Neves | |
| Author | Nunes, Beatriz Coronato | |
| Author | Jaeger, Lauren Hubert | |
| Author | Costa, Filipe Anibal Carvalho | |
| Access date | 2021-07-30T14:11:07Z | |
| Available date | 2021-07-30T14:11:07Z | |
| Document date | 2021 | |
| Citation | CALEGAR, Deiviane Aparecida et al. Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes. Parasites & Vectors, v. 14, n. 160, 13 p, 2021. | pt_BR |
| ISSN | 1756-3305 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48425 | |
| Language | eng | pt_BR |
| Publisher | BMC | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Gênero Entamoeba | pt_BR |
| Subject in Portuguese | Epidemiologia molecular | pt_BR |
| Subject in Portuguese | DNA ribossômico de subunidade pequena | pt_BR |
| Subject in Portuguese | Intra e interespecífico | pt_BR |
| Title | Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes | pt_BR |
| Type | Article | |
| DOI | 10.1186/s13071-021-04672-y | |
| Abstract | Background: Entamoeba species harbored by humans have diferent degrees of pathogenicity. The present study
explores the intra- and interspecifc diversity, phylogenetic relationships, prevalence and distribution of tetra- and
octonucleated cyst-producing Entamoeba in diferent Brazilian regions.
Methods: Cross-sectional studies were performed to collect fecal samples (n=1728) and sociodemographic data
in communities located in four Brazilian biomes: Atlantic Forest, Caatinga, Cerrado, and Amazon. Fecal samples were
subjected to molecular analysis by partial small subunit ribosomal DNA sequencing (SSU rDNA) and phylogenetic
analysis.
Results: Light microscopy analysis revealed that tetranucleated cysts were found in all the studied biomes. The
highest positivity rates were observed in the age group 6–10 years (23.21%). For octonucleated cysts, positivity
rates ranged from 1 to 55.1%. Sixty SSU rDNA Entamoeba sequences were obtained, and four diferent species were
identifed: the octonucleated E. coli, and the tetranucleated E. histolytica, E. dispar, and E. hartmanni. Novel haplotypes
(n=32) were characterized; however, new ribosomal lineages were not identifed. The Entamoeba coli ST1 subtype
predominated in Atlantic Forest and Caatinga, and the ST2 subtype was predominant in the Amazon biome. E. histol ytica was detected only in the Amazon biome. In phylogenetic trees, sequences were grouped in two groups, the frst
containing uni- and tetranucleated and the second containing uni- and octonucleated cyst-producing Entamoeba
species. Molecular diversity indexes revealed a high interspecifc diversity for tetra- and octonucleated Entamoeba
spp. (H±SD=0.9625±0.0126). The intraspecifc diversity varied according to species or subtype: E. dispar and E. histo lytica showed lower diversity than E. coli subtypes ST1 and ST2 and E. hartmanni.
Conclusions: Tetra- and octonucleated cyst-producing Entamoeba are endemic in the studied communities; E.
histolytica was found in a low proportion and only in the Amazon biome. With regard to E. coli, subtype ST2 was predominant in the Amazon biome. The molecular epidemiology of Entamoeba spp. is a feld to be further explored
and provides information with important implications for public health. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Faculdade de Medicina de Petrópolis (FMP)/ Faculdade Arthur Sá Earp Neto (FASE). Petrópolis, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal de Juiz de Fora. Faculdade de Farmácia. Campus Universitário Bairro São Pedro. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Entamoeba genus | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| Subject | Small subunit ribosomal DNA | pt_BR |
| Subject | Intra- and interspecifc | pt_BR |
| xmlui.metadata.dc.subject.ods | 05 Igualdade de gênero | |
