| Author | Sousa, Maria Carolina da Silva | |
| Author | Rossi, Maria Inês Doria | |
| Author | Evagelista, Alexandre Andrade | |
| Author | Oliveira, Gabriel Melo de | |
| Access date | 2021-07-30T14:29:28Z | |
| Available date | 2021-07-30T14:29:28Z | |
| Document date | 2021 | |
| Citation | SOUSA, Maria Carolina da Silva et al. Use of Environmental Enrichment as a Clinical Methodology During Acute Experimental Infection by Trypanosoma Cruzi. American Journal of Biomedical Research, v. 13, n. 4, 8 p, July 2021. | pt_BR |
| ISSN | 2642-1747 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48426 | |
| Language | eng | pt_BR |
| Publisher | Biomedical Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Rato | pt_BR |
| Subject in Portuguese | Bem estar animal | pt_BR |
| Subject in Portuguese | Parâmetros não invasivos | pt_BR |
| Subject in Portuguese | Enriquecimento Ambiental | pt_BR |
| Title | Use of Environmental Enrichment as a Clinical Methodology During Acute Experimental Infection by Trypanosoma Cruzi | pt_BR |
| Type | Article | |
| DOI | 10.34297/AJBSR.2021.13.001886 | |
| Abstract | One critial point in the Laboratory Animal Science (CAL) is the non-invasive evaluation of Animal Welfare (BEA). The detection (predictive or
diagnostic) of the presence of suffering in mice when used for scientific purposes is a gap. The manuscrip aim to create a technical-scientific form
to preditive presence of suffering in mice lab, based on the principle of Refinement of the 3Rs and BEA monitoring. Our methodology was based in
usual environmental enrichment the objects, the trapeze. From these observations, our study hypothesis emerged, which was, the creation of the
#1 Trap prototype and the measurement through the daily counting of the number of trapeze use during 15 minutes. Experimental acute infection
by Trypanosoma cruzi was experimental model in Swiss Webster mice. Our results described that, after division into four groups: [N] without
infection and [Inf] mice infected and without treatment. The predictive statistical difference was observed on the 11th day post infection (dpi) when
compared ([N]: 72±18.5 Events number/15min) and ([Inf]: 32±13.1 Events number/15min) (p ≤ 0.05). The 21st dpi was the most severe time point
([N]: 75±17.3 and [Inf]: 11±5.1 Events number/15min, respectively) (p ≤ 0.05). So, our results suggest the #1 Trap prototype has predictive and
monitoring capacity for experimental disease course. It makes it possible to prevent suffering and preservation of mice welfare during acute T. cruzi
experimental model. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mouse | pt_BR |
| Subject | Animal welfare | pt_BR |
| Subject | Non invasive Parameters | pt_BR |
| Subject | Environmental Enrichment | pt_BR |
| xmlui.metadata.dc.subject.ods | 01 Erradicação da pobreza | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
