| Author | Almeida-Paes, Rodrigo | |
| Author | Figueiredo-Carvalho, Maria H. G. | |
| Author | Silva, Leandro B. R. da | |
| Author | Gerfen, Gary | |
| Author | Araújo, Glauber R. de S. | |
| Author | Frases, Susana | |
| Author | Zancopé-Oliveira, Rosely M. | |
| Author | Nosanchuk, Joshua D. | |
| Access date | 2021-08-04T19:55:25Z | |
| Available date | 2021-08-04T19:55:25Z | |
| Document date | 2021 | |
| Citation | ALMEIDA-PAES, Rodrigo et al. Candida glabrata produces a melanin-like pigment that protects against stress conditions encountered during parasitism. Future microbiology, v. 16, p. 509-520, 2021 | pt_BR |
| ISSN | 1746-0913 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48491 | |
| Language | eng | pt_BR |
| Publisher | Future Medicine | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Candida glabrata produces a melanin-like pigment that protects against stress conditions encountered during parasitism | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.2217/fmb-2020-0228 | |
| Abstract | Aim: Melanin has been linked to pathogenesis in several fungi. They often produce melanin-like pigments in the presence of L-dihydroxyphenylalanine (L-DOPA), but this is poorly studied in Candida glabrata. Methods & materials: C. glabrata was grown in minimal medium with or without L-DOPA supplementation and submitted to a chemical treatment with denaturant and hot acid. Results: C. glabrata turned black when grown in the presence of L-DOPA, whereas cells grown without L-DOPA supplementation remained white. Biophysical properties demonstrated that the pigment was melanin. Melanized C. glabrata cells were effectively protected from azoles and amphotericin B, incubation at 42°C and macrophage killing. Conclusion: In the presence of L-DOPA, C. glabrata produces melanin, increases antifungal resistance and enhances host survival. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA / Universidade de São Paulo. Departamento de Microbiologia. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Biophysics. Bronx, NY, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA. | pt_BR |
| Subject | Candida glabrata | pt_BR |
| Subject | L-DOPA | pt_BR |
| Subject | Melanin | pt_BR |
| Subject | Survival | pt_BR |
| Subject | Virulence | pt_BR |
