| Author | Corrêa, Jessica Badolato | |
| Author | Carvalho, Fabiana Rabe | |
| Author | Paiva, Iuri Amancio | |
| Author | Macedo, Débora Familiar | |
| Author | Dias, Helver Gonçalves | |
| Author | Corrêa, Alex Pauvolid | |
| Author | Santos, Caroline Fernandes | |
| Author | Lima, Monique da Rocha Queiroz | |
| Author | Gandini, Mariana | |
| Author | Silva, Andréa Alice | |
| Author | Cavalcanti, Silvia Maria Baeta | |
| Author | Oliveira, Solange Artimos de | |
| Author | Vianna, Renata Artimos de Oliveira | |
| Author | Azeredo, Elzinandes Leal de | |
| Author | Cardoso, Claudete Aparecida Araújo | |
| Author | Grifoni, Alba | |
| Author | Sette, Alessandro | |
| Author | Weiskopf, Daniela | |
| Author | PInto, Luzia Maria de Oliveira | |
| Access date | 2021-08-09T18:51:57Z | |
| Available date | 2021-08-09T18:51:57Z | |
| Document date | 2021 | |
| Citation | CORRÊA, Jessica Badolato et al. Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children. Frontiers in immunology, v. 12, Article 610456, p. 1-17, Feb. 2021. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48518 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Zika | pt_BR |
| Subject in Portuguese | Células T | pt_BR |
| Subject in Portuguese | Memória | pt_BR |
| Subject in Portuguese | Gravidez | pt_BR |
| Subject in Portuguese | Síndrome congênita do Zika | pt_BR |
| Title | Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children | pt_BR |
| Type | Article | |
| DOI | 10.3389/fimmu.2021.610456 | |
| Abstract | Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in
healthy adults. In newborns, it can occasionally lead to a spectrum of malformations,
the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a
history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues,
we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to
the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants
affected by neurological complications or asymptomatic ones.
Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell
responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools
(CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed
an increased CD4 and CD8 T-cell responses in mothers compared to children. The
degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers,
and children, while in CD8 T-cells, low responses were detected in these study groups.
The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4
ZIKV MP.
Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell
immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with
the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities
of CD8 T cells are markedly demonstrated in the early stages of infection, but less
detected in the disease resolution phase, when the virus has already been eliminated. The Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in
healthy adults. In newborns, it can occasionally lead to a spectrum of malformations,
the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a
history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues,
we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to
the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants
affected by neurological complications or asymptomatic ones.
Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell
responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools
(CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed
an increased CD4 and CD8 T-cell responses in mothers compared to children. The
degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers,
and children, while in CD8 T-cells, low responses were detected in these study groups.
The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4
ZIKV MP.
Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell
immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with
the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities
of CD8 T cells are markedly demonstrated in the early stages of infection, but less
detected in the disease resolution phase, when the virus has already been eliminated. The Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in
healthy adults. In newborns, it can occasionally lead to a spectrum of malformations,
the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a
history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues,
we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to
the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants
affected by neurological complications or asymptomatic ones.
Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell
responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools
(CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed
an increased CD4 and CD8 T-cell responses in mothers compared to children. The
degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers,
and children, while in CD8 T-cells, low responses were detected in these study groups.
The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4
ZIKV MP.
Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell
immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with
the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities
of CD8 T cells are markedly demonstrated in the early stages of infection, but less
detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers’ T cells to ZIKV MPs do not appear to be related to their children’s
clinical outcome. There was also no marked difference in the T cell responses to ZIKV
MP between children affected or not with CZS. These data still need to be investigated,
including the evaluation of the response of CD8 T cells to other ZIKV peptides. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Escola de Medicina. Laboratório Multiusuário para Pesquisa em Nefrologia e Ciências Médicas. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Department of Veterinary Integrative Biosciences, Texas A&M University, College Station. TX, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Escola de Medicina. Laboratório Multiusuário para Pesquisa em Nefrologia e Ciências Médicas. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Laboratório de Diagnóstico Virológico. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Escola de Medicina. Departamento Materno Infantil. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Escola de Medicina. Departamento Materno Infantil. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Escola de Medicina. Laboratório Multiusuário para Pesquisa em Nefrologia e Ciências Médicas. Niterói, RJ, Brasil / Universidade Federal Fluminense. Escola de Medicina. Departamento Materno Infantil. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI). San Diego, CA, USA. | pt_BR |
| Affilliation | Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI). San Diego, CA, USA / Division of Infectious Diseases and Global Public Health, Department of Medicine, University of California, San Diego, San Diego, CA, USA. | pt_BR |
| Affilliation | Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI). San Diego, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Zika | pt_BR |
| Subject | T cells | pt_BR |
| Subject | Memory | pt_BR |
| Subject | Pregnancy | pt_BR |
| Subject | Congenital Zika syndrome (CZS) | pt_BR |
