| Author | Pitanga, Thassila Nogueira | pt_BR |
| Author | Santana, Sânzio Silva | pt_BR |
| Author | Zanette, Dalila Lucíola | pt_BR |
| Author | Guarda, Caroline Conceição | pt_BR |
| Author | Santiago, Rayra P. | pt_BR |
| Author | Maffili, Vitor Valério | pt_BR |
| Author | Lima, Jonilson B. | pt_BR |
| Author | Carvalho, Graziele Q. | pt_BR |
| Author | Ribeiro Filho, Jaime | pt_BR |
| Author | Ferreira, Junia R. D. | pt_BR |
| Author | Aleluia, Milena M. | pt_BR |
| Author | Nascimento, Valma M. L. | pt_BR |
| Author | Carvalho, Magda O. S. | pt_BR |
| Author | Lyra, Isa M. | pt_BR |
| Author | Borges, Valéria de Matos | pt_BR |
| Author | Oliveira, Ricardo R. | pt_BR |
| Author | Gonçalves, Marilda de Souza | pt_BR |
| Access date | 2021-08-30T14:03:07Z | |
| Available date | 2021-08-30T14:03:07Z | |
| Document date | 2021 | |
| Citation | PITANGA, Thassila Nogueira et al. Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells. Inflammation Research, v. 70, p. 823-834, 1 July 2021. | pt_BR |
| ISSN | 1023-3830 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48825 | |
| Sponsorship | Brazilian National Council of Research (CNPq) (311888/2013-5) (M.S.G.) | pt_BR |
| Sponsorship | Foundation of Research and Extension of Bahia (FAPESB) (3626/2013, 1431040053063, and 9073/2007) | pt_BR |
| Sponsorship | PPSUS/FAPESB (020/2013 EFP00007295) | pt_BR |
| Sponsorship | Instituto Nacional de Ciência e Tecnologia do Sangue (INCTs) and MCD/CNPq/MS-SCTIE-DECIT (409800/2006-6) | pt_BR |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Anemia falciforme | pt_BR |
| Subject in Portuguese | Inflamassomos | pt_BR |
| Subject in Portuguese | Leucócitos mononucleares | pt_BR |
| Subject in Portuguese | Hidroxiureia | pt_BR |
| Subject in Portuguese | Reação em cadeia da polimerase | pt_BR |
| Title | Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells | en_US |
| Type | Article | |
| DOI | 10.1007/s00011-021-01461-2 | pt_BR |
| Abstract | Objective and design: This study tested the hypothesis that sickle red blood cell (SS-RBC) can induce inflammasome NLRP3 components gene expression in peripheral blood mononuclear cells (PBMCs) as well as interleukin-1β (IL-1β) and leukotriene B4 (LTB4) production. Additionally, we investigated the effect of hydroxyurea (HU) treatment in these inflammatory markers. Methods: PBMCs from healthy donors (AA-PBMC) were challenged with intact and lysed RBCs from SCA patients (SS-RBC) and from healthy volunteers (AA-RBC). NLRP3, IL-1β, IL-18 and Caspase-1 gene expression levels were assessed by quantitative PCR (qPCR). IL-1β protein levels and LTB4 were measured by ELISA. Results: We observed that lysed SS-RBC induced the expression of inflammasome NLRP3 components, but this increase was more prominent for CASP1 and IL18 expression levels. Moreover, we observed that intact SS-RBC induced higher production of IL-1β and LTB4 than lysed SS-RBC. Although SCA patients treated with HU have a reduction in NLRP3 gene expression and LTB4 production, this treatment did not modulate the expression of other inflammasome components or IL-1β production. Conclusions: Thus, our data suggest that caspase-1, IL-1β and IL-18 may contribute to the inflammatory status observed in SCA and that HU treatment may not interfere in this inflammatory pathway. | en |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Católica do Salvador. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Católica do Salvador. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Oeste da Bahia. Barreiras, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Santa Cruz. Ilhéus, BA, Brasil. | pt_BR |
| Affilliation | Fundação de Hematologia e Hemoterapia da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Sistema Universitário de Saúde. Complexo Hospitalar Universitário Professor Edgard Santos. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
| Subject | Sickle cell anemia | en |
| Subject | NLRP3-inflammasome | en |
| Subject | Hemolysis | en |
| Subject | Hydroxyurea | en |
| Subject | Heme | en |
| e-ISSN | 1420-908X | pt_BR |
