Author | Hauser-Davis, Rachel Ann | |
Author | Lavradas, Raquel T. | |
Author | Monteiro, Fernanda | |
Author | Rocha, Rafael Christian C. | |
Author | Bastos, Frederico F. | |
Author | Araújo, Gabriel F. | |
Author | Sales Junior, Sidney F. | |
Author | Bordon, Isabella C. | |
Author | Correia, Fábio V. | |
Author | Saggioro, Enrico M. | |
Author | Saint`Pierre, Tatiana D. | |
Author | Godoy, José M. | |
Access date | 2021-09-03T18:50:58Z | |
Available date | 2021-09-03T18:50:58Z | |
Document date | 2020 | |
Citation | HAUSER-DAVIS, Rachel Ann et al. Biochemical metal accumulation effects and metalloprotein metal detoxification in environmentally exposed tropical Perna perna mussels. Ecotoxicology and Envionmental Safety, v. 208, 111589, 11 p, Nov. 2020. | pt_BR |
ISSN | 0147-6513 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/48962 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | |
Subject in Portuguese | Estresse oxidativo | pt_BR |
Subject in Portuguese | Bivalve | pt_BR |
Subject in Portuguese | Desintoxicação de metal | pt_BR |
Subject in Portuguese | Metalômica | pt_BR |
Title | Biochemical metal accumulation effects and metalloprotein metal detoxification in environmentally exposed tropical Perna perna mussels | pt_BR |
Type | Article | |
DOI | 10.1016/j.ecoenv.2020.111589 | |
Abstract | Marine bivalves have been widely applied as environmental contamination bioindicators, although studies
concerning tropical species are less available compared to temperate climate species. Assessments regarding
Perna perna mytilid mussels, in particular, are scarce, even though this is an extremely important species in
economic terms in tropical countries, such as Brazil. To this end, Perna perna mytilids were sampled from two
tropical bays in Southeastern Brazil, one anthropogenically impacted and one previously considered a reference
site for metal contamination. Gill metallothionein (MT), reduced glutathione (GSH), carboxylesterase (CarbE)
and lipid peroxidation (LPO) were determined by UV− vis spectrophotometry, and metal and metalloid contents
were determined by inductively coupled plasma mass spectrometry (ICP− MS). Metalloprotein metal detoxifi cation routes in heat-stable cellular gill fractions were assessed by size exclusion high performance chroma tography (SEC− HPLC) coupled to an ICP− MS. Several associations between metals and oxidative stress
endpoints were observed at all four sampling sites through a Principal Component Analysis. As, Cd, Ni and Se
contents, in particular, seem to directly affect CarbE activity. MT is implicated in playing a dual role in both
metal detoxification and radical oxygen species scavenging. Differential SEC− HPLC− ICP− MS metal-binding
profiles, and, thus, detoxification mechanisms, were observed, with probable As-, Cu- and Ni-GSH complexa tion and binding to low molecular weight proteins. Perna perna mussels were proven adequate tropical bio indicators, and further monitoring efforts are recommended, due to lack of data regarding biochemical metal
effects in tropical species. Integrated assessments, as performed herein demonstrate, are invaluable in evaluating
contaminated aquatic environments, resulting in more accurate ecological risk assessments. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantâra Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pos-graduação em Saúde Pública e Meio Ambiente. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pos-graduação em Saúde Pública e Meio Ambiente. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal do Estado do Rio de Janeiro -UNIRIO. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Fundação Oswaldo Cruz. Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Oxidative stress | pt_BR |
Subject | Bivalve | pt_BR |
Subject | Metal detoxification | pt_BR |
Subject | Metallomics | pt_BR |