| Author | Dias, Beatriz do Carmo | |
| Author | Mota, Fabio Faria da | |
| Author | Jurelevicius, Diogo | |
| Author | Seldin, Lucy | |
| Access date | 2021-09-05T12:03:23Z | |
| Available date | 2021-09-05T12:03:23Z | |
| Document date | 2021 | |
| Citation | DIAS, Beatriz do Carmo et al. Genetics and regulation of nitrogen fixation in Paenibacillus brasilensis PB24. Microbiological Research, v. 243, 126647, 9 p, 2021. | pt_BR |
| ISSN | 0944-5013 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/48982 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Paenibacillus brasilensis | pt_BR |
| Subject in Portuguese | Fixação de nitrogênio | pt_BR |
| Subject in Portuguese | nif genes | pt_BR |
| Subject in Portuguese | Nitrogenase | pt_BR |
| Subject in Portuguese | Regulamento | pt_BR |
| Title | Genetics and regulation of nitrogen fixation in Paenibacillus brasilensis PB24 | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.micres.2020.126647 | |
| Abstract | Biological nitrogen fixation (BNF), performed by diazotrophic prokaryotes, is responsible for reducing dinitrogen
(N2) present in the biosphere into biologically available forms of nitrogen. Paenibacillus brasilensis PB24 is a
diazotrophic Gram-positive bacterium and is considered ecologically and industrially important because it is able
to produce antimicrobial substances and 2,3-butanediol. However, the genetics and regulation of its nitrogen
fixing (nif) genes have never been assessed so far. Therefore, the present study aimed to (i) identify the structural
and regulatory genes related to BNF in the PB24 genome, (ii) perform comparative genomics analysis of the nif
operon among different Paenibacillus species and (iii) study the expression of these genes in the presence and
absence of NH4. Strain PB24 showed a nif operon composed of nine genes (nifBHDKENXhesAV), with a conserved
synteny (with small variations) among the Paenibacillus species evaluated. BNF regulatory genes, glnK and amtB
(encoding GlnK signal transduction protein and AmtB transmembrane protein, respectively) and glnR and glnA
genes (encoding the transcription factor GlnR and glutamine synthetase) were found in the PB24 genome.
Primers were designed for qPCR amplification of the nitrogenase structural (nifH, nifD and nifK) and regulatory
(glnA and amtB) BNF genes. The structural gene expression in PB24 was up- and downregulated in the absence
and presence of NH4, respectively. The gene expression levels indicated a GlnR-mediated repression of genes
associated with ammonium import (amtBglnK) and BNF (nif genes). Additionally, the regulatory mechanism of
GlnR in P. brasilensis PB24 differed from the other Paenibacillus evaluated, considering the different distribution
of binding sites recognized by GlnR. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Genetica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Genetica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Genetica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Paenibacillus brasilensis | pt_BR |
| Subject | Nitrogen fixation | pt_BR |
| Subject | nif genes | pt_BR |
| Subject | Nitrogenase | pt_BR |
| Subject | Regulation | pt_BR |
| Embargo date | 2023 | |
