| Author | Rodart, Itatiana F. | |
| Author | Pares, Madalena M. | |
| Author | Mendes, Aline | |
| Author | Accardo, Camila M. | |
| Author | Martins, João R. M. | |
| Author | Silva, Cleidenice B. | |
| Author | Carvalho, Fabrício O. | |
| Author | Barreto, José A. | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Tersariol, Ivarne L. S. | |
| Author | Nader, Helena B. | |
| Access date | 2021-09-22T18:49:54Z | |
| Available date | 2021-09-22T18:49:54Z | |
| Document date | 2021 | |
| Citation | RODART, Itatiana F. et al. Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors. Molecules, 2021. | pt_BR |
| ISSN | 1420-3049 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49136 | |
| Sponsorship | São Paulo Research Foundation-FAPESP (http://www.
fapesp.br, Accessed on 30 April 2021) Grants 2015/03964-6; awarded to H.B.N. and I.L.S.T.; I.F.R.
and C.M.A. were recipients of scholarship grants from Coordenação de Aperfeiçoamento de Pessoal
de Nível Superior, Brasil, CAPES (http://www.capes.gov.br/, Accessed on 30 April 2021); H.B.N.
and I.L.S.T. are Productivity Fellows from Conselho Nacional de Desenvolvimento Científico e
Tecnológico, Brasil, CNPq (https://www.gov.br/cnpq/pt-br, Accessed on 30 April 2021). | pt_BR |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | RNA | pt_BR |
| Subject in Portuguese | Progressão da Doença | pt_BR |
| Subject in Portuguese | Fibrose | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Doadores de sangue | pt_BR |
| Title | Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3390/molecules26133892 | |
| Abstract | Background: The disease caused by hepatitis C virus (HCV) is asymptomatic, silent, and
progressive liver disease. In HCV-infected patients the increase in serum HA is associated with
the development of hepatic fibrosis and disease progression. Methods: HCV-RNA detection was
performed in all serological samples of blood donors that tested positive using HCV Ultra ELISA.
Determination of hyaluronan (HA) was performed in positive HCV samples using ELISA-like
fluorometric method. The HA content was compared to HCV viral load, genotype of the virus, liver
fibrosis as well as ALT and GGT liver biomarkers. Results: Persistently normal ALT (<40 U/L) and
GGT (<50 U/L) serum levels were detected in 75% and 69% of the HCV-Infected blood donors,
respectively. Based on ROC analysis, the HA value < 34.2 ng/mL is an optimal cut-off point to
exclude HCV viremia (specificity = 91%, NPV = 99%). Applying HA value 34.2 ng/mL significant
liver fibrosis ( F2) can be estimated in 46% of the HCV-infected blood donors. HA serum level
( 34.2 ng/mL) associated with a high ALT level (>40 U/mL) can correctly identify HCV infection
and probable liver fibrosis (sensitivity = 96% and specificity = 90%) in asymptomatic blood donors.
Conclusions: A high level of HA ( 34.2 ng/mL) in association with ALT ( 40 U/L) in serum can
provide a good clinical opportunity to detect HCV-infected asymptomatic persons that potentially
require a liver biopsy confirmation and antiviral treatment to prevent the development of advanced
liver fibrosis or cirrhosis. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil. | pt_BR |
| Affilliation | Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil. | pt_BR |
| Affilliation | Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hepatites Virais. Salvador, BA, Brasil. | pt_BR |
| Subject | Serum hyaluronan | pt_BR |
| Subject | Diagnostic accuracy | pt_BR |
| Subject | Liver fibrosis | pt_BR |
| Subject | Liver enzymes | pt_BR |
| Subject | HCV infection | pt_BR |
| Subject | Blood donors | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
