| Author | Rasinhas, Arthur da Costa | |
| Author | Jácome, Fernanda Cunha | |
| Author | Caldas, Gabriela Cardoso | |
| Author | Almeida, Ana Luisa Teixeira de | |
| Author | Silva, Marcos Alexandre Nunes da | |
| Author | Souza, Daniel Dias Coutinho de | |
| Author | Paulino, Amanda Carlos | |
| Author | Bandeira, Derick Mendes | |
| Author | Leonardo, Raphael | |
| Author | Nunes, Priscila Conrado Guerra | |
| Author | Borges, Ronaldo Mohana | |
| Author | Barth, Otrud Monika | |
| Author | Santos, Flavia Barreto dos | |
| Author | Vieira, Debora Ferreira Barreto | |
| Access date | 2021-10-12T20:25:41Z | |
| Available date | 2021-10-12T20:25:41Z | |
| Document date | 2021 | |
| Citation | RASINHAS, Arthur da Costa et al. Morphological Aspects and Viremia Analysis of BALB/c Murine Model Experimentally Infected with Dengue Virus Serotype 4. Viruses, v. 13, n.1954, p. 1-21, Sept. 2021. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49351 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | DENV-4 | pt_BR |
| Subject in Portuguese | Camundongos Endogâmicos BALB C | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Subject in Portuguese | Pulmão | pt_BR |
| Subject in Portuguese | Coração | pt_BR |
| Subject in Portuguese | Histopatologia | pt_BR |
| Subject in Portuguese | Ultraestrutura | pt_BR |
| Title | Morphological Aspects and Viremia Analysis of BALB/c Murine Model Experimentally Infected with Dengue Virus Serotype 4 | pt_BR |
| Type | Article | |
| DOI | 10.3390/v13101954 | |
| Abstract | Ever since its brief introduction in the Brazilian territory in 1981, dengue virus serotype
4 (DENV-4) remained absent from the national epidemiological scenario for almost 25 years. The
emergence of DENV-4 in 2010 resulted in epidemics in most Brazilian states. DENV-4, however,
remains one of the least studied among the four DENV serotypes. Despite being known as a mild
serotype, DENV-4 is associated with severe cases and deaths and deserves to be investigated; however,
the lack of suitable experimental animal models is a limiting factor for pathogenesis studies. Here,
we aimed to investigate the susceptibility and potential tropism of DENV-4 for liver, lung and heart
of an immunocompetent mice model, and to evaluate and investigate the resulting morphological
and ultrastructural alterations upon viral infection. BALB/c mice were inoculated intravenously
with non-neuroadapted doses of DENV-4 isolated from a human case. The histopathological analysis
of liver revealed typical alterations of DENV, such as microsteatosis, edema and vascular congestion,
while in lung, widespread areas of hemorrhage and interstitial pneumonia were observed. While
milder alterations were present in heart, characterized by limited hemorrhage and discrete presence
of inflammatory infiltrate, the disorganization of the structure of the intercalated disc is of particular
interest. DENV-4 RNA was detected in liver, lung, heart and serum of BALB/c mice through
qRT-PCR, while the NS3 viral protein was observed in all of the aforementioned organs through
immunohistochemistry. These findings indicate the susceptibility of the model to the serotype and
further reinforce the usefulness of BALB/c mice in studying the many alterations caused by DENV. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biotecnologia e Bioengenharia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral . Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | DENV-4 | pt_BR |
| Subject | BALB/c mice | pt_BR |
| Subject | Liver | pt_BR |
| Subject | Heart | pt_BR |
| Subject | Hstopathology | pt_BR |
| Subject | Ultrastructure | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
