| Author | Licá, Irrla Correia Lima | |
| Author | Soares, Alexandra Martins Santos | |
| Author | Ferreira, André Teixeira Silva | |
| Author | Perales, Jonas | |
| Author | Cunha, Ivo Alexandre Leme | |
| Author | Souza, Pedro Filho Noronha | |
| Author | Costa Junior, Livio Martins | |
| Access date | 2021-11-04T19:11:08Z | |
| Available date | 2021-11-04T19:11:08Z | |
| Document date | 2021 | |
| Citation | LICÁ, Irrla Correia Lima et al. In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus. Veterinary Parasitology, v. 292, 109399, 5 p, 2021. | pt_BR |
| ISSN | 0304-4017 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49671 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Proteínas de defesa vegetal | pt_BR |
| Subject in Portuguese | Nematóide | pt_BR |
| Subject in Portuguese | Proteoma | pt_BR |
| Subject in Portuguese | Exsheathment | pt_BR |
| Subject in Portuguese | Incubação de ovos | pt_BR |
| Title | In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetpar.2021.109399 | |
| Abstract | Nematodes develop resistance to the most common commercially available drugs. The aim of this study was to
identify and evaluate the action of protein exudates from Mimosa caesalpiniifolia, Leucaena leucocephala, Acacia
mangium, and Stylosanthes capitata seeds on the gastrointestinal nematode Haemonchus contortus. The exuded
proteins were precipitated, dialyzed, lyophilized, and assessed for their effect on egg hatching and artificial larval
exsheathment inhibition. Proteome analysis of the protein extracts was also performed. Although no egghatching
inhibition was observed, all exudates showed efficacy in inhibiting the larval exsheathment of
H. contortus larvae with an EC50 varying from 0.61 to 0.26 mg P mL 1. Proteomic analysis revealed the presence
of proteases, protease inhibitors, chitinases, and lectins among other proteins in the exudates. Most of the exuded
proteins belong to the oxidative stress/plant defense and energy/carbohydrate metabolism functional clusters.
This study concluded that the bioactive proteins from different classes exuded by seeds of M. caesalpiniifolia,
L. leucocephala, A. mangium, and S. capitata show stage-specific inhibition against H. contortus. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Programa de Pós-Graduação em Ciências da Saúde. São Luís, MA, Brasil / Universidade Federal do Maranhão. Laboratório de Bioquímica Vegetal,. São Luís, MA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Programa de Pós-Graduação em Ciências da Saúde. São Luís, MA, Brasil / Universidade Federal do Maranhão. Laboratório de Bioquímica Vegetal,. São Luís, MA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Laboratório de Parasitologia Aplicada. São Luís, MA, Brasil. | pt_BR |
| Affilliation | University of Nebraska Lincoln. Center for Virology. LIncoln, NE, USA. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Programa de Pós-Graduação em Ciências da Saúde. São Luís, MA, Brasil / Universidade Federal do Maranhão. Laboratório de Controle de Parasitas. São Luís, MA, Brasil. | pt_BR |
| Subject | Plant defense proteins | pt_BR |
| Subject | Nematode | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Exsheathment | pt_BR |
| Subject | Egg Hatching | pt_BR |
| e-ISSN | 1873-2550 | |
| Embargo date | 2023 | |
