| Author | Sarmento, Sylvia Kahwage | |
| Author | Andrade, Juliana da Silva Ribeiro de | |
| Author | Miagostovich, Marize Pereira | |
| Author | Fumian, Tulio Machado | |
| Access date | 2021-11-05T13:42:50Z | |
| Available date | 2021-11-05T13:42:50Z | |
| Document date | 2021 | |
| Citation | SARMENTO, Sylvia Kahwage et al. Virological and epidemiological features of norovirus infections in Brazil, 2017-2018. Viruses, v. 13, n. 9, p. 1-16, 30 Aug. 2021. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49706 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Norovirus | pt_BR |
| Subject in Portuguese | Genotipagem dupla | pt_BR |
| Subject in Portuguese | Epidemiologia molecular | pt_BR |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | Carga viral | pt_BR |
| Subject in Portuguese | Clínica | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Virological and epidemiological features of norovirus infections in Brazil, 2017-2018 | pt_BR |
| Type | Article | |
| DOI | 10.3390/v13091724 | pt_BR |
| Abstract | Noroviruses are considered an important cause of acute gastroenteritis (AGE) across all age groups. Here, we investigated the incidence of norovirus, genotypes circulation, and norovirus shedding in AGE stool samples from outpatients in Brazil. During a two-year period, 1546 AGE stool samples from ten Brazilian states were analyzed by RT-qPCR to detect and quantify GI and GII noroviruses. Positive samples were genotyped by dual sequencing using the ORF1/2 junction region. Overall, we detected norovirus in 32.1% of samples, with a massive predominance of GII viruses (89.1%). We also observed a significant difference between the median viral load of norovirus GI (3.4×105 GC/g of stool) and GII (1.9×107 GC/g). The most affected age group was children aged between 6 and 24 m old, and norovirus infection was detected throughout the year without marked seasonality. Phylogenetic analysis of partial RdRp and VP1 regions identified six and 11 genotype combinations of GI and GII, respectively. GII.4 Sydney[P16] was by far the predominant genotype (47.6%), followed by GII.2[P16], GII.4 Sydney[P31], and GII.6[P7]. We detected, for the first time in Brazil, the intergenogroup recombinant genotype GIX.1[GII.P15]. Our study contributes to the knowledge of norovirus genotypes circulation at the national level, reinforcing the importance of molecular surveillance programs for future vaccine designs. | en |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Norovirus | en |
| Subject | Dual genotyping | en |
| Subject | Molecular epidemiology | en |
| Subject | Genetic diversity | en |
| Subject | Viral load | en |
| Subject | Clinical | en |
| Subject | Brazil | en |
| e-ISSN | 1999-4915 | pt_BR |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
