| Author | Robert, Anny Waloski | |
| Author | Stimamiglio, Marco Augusto | |
| Access date | 2021-11-08T19:46:00Z | |
| Available date | 2021-11-08T19:46:00Z | |
| Document date | 2020 | |
| Citation | ROBERT, Anny Waloski; STIMAMIGLIO, Marco Augusto. The secretome from embryonic stem cell cardiomyogenesis: Same signals, different cellular feedbacks. Journal of Cellular Physiology, v. 236, n. 2, p. 971-980, 2020. | pt_BR |
| ISSN | 1097-4652 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49732 | |
| Language | por | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Secretome | pt_BR |
| Title | The secretome from embryonic stem cell cardiomyogenesis: same signals, different cellular feedbacks | pt_BR |
| Type | Article | |
| DOI | 10.1002/jcp.29907 | |
| Abstract | Ischemic heart diseases are a global health problem that requires the search for alternative therapies to the current treatments. Thus, an understanding of how cardiomyogenic signals can affect cellular behavior would allow us to create strategies to improve the cell recovery in damaged tissues. In this study, we aimed to evaluate the effects of the conditioned medium (CM), collected at different time points during in vitro cardiomyogenesis of human embryonic stem cells (hESCs), to direct cell behavior. We assayed different cell types to demonstrate noncytotoxic effects from the collected CM and that the CM obtained at initial time points of cardiomyogenic differentiation could promote the cell proliferation. Otherwise, the secretome derived from cardiac committed cells during cardiomyogenesis was unable to improve angiogenesis or migration in endothelial cells, and ineffective to stimulate the differentiation of cardioblasts or increase the differentiation efficiency of hESC. Therefore, we demonstrated that the effectiveness of the CM response varies depending on the cell type and the differentiation step of hESC-derived cardiomyocytes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Subject | Myocytes Cardiac | pt_BR |
| Subject | Cell Differentiation | pt_BR |
| Subject | Culture Media Conditioned | pt_BR |
| Subject | Embryonic Stem Cells | pt_BR |
| Subject in Spanish | Miocitos Cardíacos | pt_BR |
| Subject in Spanish | Diferenciación Celular | pt_BR |
| Subject in Spanish | Medios de Cultivo Condicionados | pt_BR |
| Subject in Spanish | Células Madre Embrionarias | pt_BR |
| Subject in French | Myocytes cardiaques | pt_BR |
| Subject in French | Différenciation cellulaire | pt_BR |
| Subject in French | Milieux de culture conditionnés | pt_BR |
| Subject in French | Cellules souches embryonnaires | pt_BR |
| DeCS | Miócitos Cardíacos | pt_BR |
| DeCS | Diferenciação Celular | pt_BR |
| DeCS | Meios de Cultivo Condicionados | pt_BR |
| DeCS | Células-Tronco Embrionárias | pt_BR |
