| Author | Alvarenga, Larissa Magalhães | |
| Author | Cardenas, Guillermo Andrés Cerquera | |
| Author | Jiacomini, Isabella Gizzi | |
| Author | Ramirez, Marcel Ivan | |
| Access date | 2021-11-09T12:46:37Z | |
| Available date | 2021-11-09T12:46:37Z | |
| Document date | 2021 | |
| Citation | ALVARENGA, Larissa Magalhães et al. A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism. Toxicology Letters, n. 350, p. 202–212, 2021. | pt_BR |
| ISSN | 0378-4274 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49741 | |
| Language | por | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.toxlet.2021.07.014 | |
| Abstract | Envenomation by the Loxosceles genus spiders is a recurring health issue worldwide and specially in the
Americas. The physiopathology of the envenomation is tightly associated to the venom’s rich toxin
composition, able to produce a local dermonecrotic lesion that can evolve systemically and if worsened,
might result in multiple organ failure and lethality. The cellular and molecular mechanisms involved with
the physiopathology of Loxoscelism are not completely understood, however, the venom’s Phospholipases D (PLDs) are known to trigger membrane injury in various cell types. Here, we report for the first
time the Loxosceles venom’s ability to stimulate the production of extracellular vesicles (EVs) in various
human cell lineages. Components of the Loxosceles venom were also detectable in the cargo of these
vesicles, suggesting that they may be implicated in the process of extracellular venom release. EVs from
venom treated cells exhibited phospholipase D activity and were able to induce in vitro hemolysis in
human red blood cells and alter the HEK cell membranes’ permeability. Nonetheless, the PLD activity was
inhibited when an anti-venom PLDs monoclonal antibody was co-administered with the whole venom.
In summary, our findings shed new light on the mechanisms underlying cellular events in the context of
loxoscelism and suggest a crucial role of EVs in the process of envenomation. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. EVAHPI – Extracellular Vesicles and Host-Parasite Interactions Research Group. Curitiba, PR, Brasil. | pt_BR |
| Subject | Loxosceles | pt_BR |
| Subject | Brown Recluse Spider | pt_BR |
| Subject | Phospholipases D | pt_BR |
| Subject | Group II Phospholipases A2 | pt_BR |
| Subject | Extracellular Vesicles | pt_BR |
| Subject | Envenomation | pt_BR |
| Subject in Spanish | Araña Reclusa Parda | pt_BR |
| Subject in Spanish | Fosfolipasas A2 Grupo II | pt_BR |
| Subject in Spanish | Vesículas Extracelulares | pt_BR |
| Subject in Spanish | Envenenamiento | pt_BR |
| Subject in French | Araignée recluse brune | pt_BR |
| Subject in French | Group II Phospholipases A2 | pt_BR |
| Subject in French | Vésicules extracellulaires | pt_BR |
| Subject in French | empoisonnement | pt_BR |
| DeCS | Aranha Marrom Reclusa | pt_BR |
| DeCS | Fosfolipases A2 do Grupo II | pt_BR |
| DeCS | Vesículas Extracelulares | pt_BR |
| DeCS | Envenenamento | pt_BR |
