| Author | Ribeiro, Annanda Lyra | |
| Author | Bassai, Letícia Werzel | |
| Author | Robert, Anny Waloski | |
| Author | Machado, Thiago Neves | |
| Author | Bezerra Júnior, Arandi Ginane | |
| Author | Horinouchi, Cintia Delai da Silva | |
| Author | Aguiar, Alessandra Melo de | |
| Access date | 2021-11-09T18:48:51Z | |
| Available date | 2021-11-09T18:48:51Z | |
| Document date | 2021 | |
| Citation | RIBEIRO, Annanda Lyra et al. Bismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells. Toxicology in Vitro, v. 77, n. 105248, p. 1–10, 2021. | pt_BR |
| ISSN | 0887-2333 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/49753 | |
| Language | por | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Bismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.tiv.2021.105248 | |
| Abstract | Bismuth-based nanoparticles (BiNPs) have attracted attention for their potential biomedical applications.
However, there is a lack of information concerning their interaction with biological systems. In this study, it was
investigated the effect of physically synthesized BiNPs to human adipose-derived stem cells (ADSCs). We first
evaluated the influence of BiNPs on cell viability, cell morphology, mitochondrial function and cell proliferation.
Further, the impact of BiNPs on adipogenic differentiation was also explored. Cytotoxicity assays have demonstrated that BiNPs did not reduce relative cell viability of ADSC except at the highest tested concentration (345 μg/ml). Analysis of cell morphology performed by transmission electron microscopy confirmed that BiNPs induced cell damage only at a high concentration (302.24 μg/ml), equivalent to IC50 concentration. Moreover, BiNPs exposure increased the expression of the cell proliferation marker Ki-67 and the incorporation of the thymidine analogue EdU into cell DNA, suggesting that these nanoparticles could be stimulating ADSC proliferation. BiNPs also increased the mitochondrial membrane potential. Furthermore, BiNPs reduced ADSC adipogenic differentiation as measured by lipid droplet accumulation and mRNA expression levels of the specific adipogenesis biomarkers PPARγ, C/EPBɑ and FABP4. Thus, BiNPs affect the nonspecific (viability, proliferation and mitochondrial activity) and specific (adipogenesis) cellular mechanisms of ADSCs. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Laboratório de Cultivo de Eucariotos. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Tecnológica Federal do Paraná. Laboratório Fotonanobio. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Tecnológica Federal do Paraná. Laboratório Fotonanobio. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil. | pt_BR |
| Subject | Nanoparticles | pt_BR |
| Subject | Bismuth | pt_BR |
| Subject | Mesenchymal Stem Cells | pt_BR |
| Subject | Cell Differentiation | pt_BR |
| Subject in Spanish | Nanopartículas | pt_BR |
| Subject in Spanish | Células Madre Mesenquimatosas | pt_BR |
| Subject in Spanish | Diferenciación Celular | pt_BR |
| Subject in French | Nanoparticules | pt_BR |
| Subject in French | Cellules souches mésenchymateuses | pt_BR |
| Subject in French | Différenciation cellulaire | pt_BR |
| DeCS | Nanopartículas | pt_BR |
| DeCS | Bismuto | pt_BR |
| DeCS | Células-Tronco Mesenquimais | pt_BR |
| DeCS | Diferenciação Celular | pt_BR |
