Author | Mateus, Jackeline Rossetti | |
Author | Dal’Rio, Isabel | |
Author | Jurelevicius, Diogo | |
Author | Mota, Fabio Faria da | |
Author | Marques, Joana Montezano | |
Author | Ramos, Rommel Thiago Juca | |
Author | Silva, Artur Luiz da Costa da | |
Author | Gagliardi, Paulo Roberto | |
Author | Seldin, Lucy | |
Access date | 2021-11-11T18:05:03Z | |
Available date | 2021-11-11T18:05:03Z | |
Document date | 2021 | |
Citation | MATEUS, Jackeline Rossetti et al. Bacillus velezensis T149-19 and Bacillus safensis T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato. Agriculture, v. 11, 1046, p. 1 -14, Oct. 2021. | pt_BR |
ISSN | 2077-0472 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49778 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Bacillus velezensis | pt_BR |
Subject in Portuguese | Bacillus safensis | pt_BR |
Subject in Portuguese | Plenodomus destruens | pt_BR |
Subject in Portuguese | Doença da podridão dos pés | pt_BR |
Subject in Portuguese | Biocontrole | pt_BR |
Subject in Portuguese | Batata doce | pt_BR |
Title | Bacillus velezensis T149-19 and Bacillus safensis T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/ agriculture11111046 | |
Abstract | Sweet potato (Ipomoea batatas) tuberous roots are used for human consumption, animal
feed, and many industrial products. However, the crop is susceptible to various pests and diseases,
including foot rot disease caused by the phytopathogenic fungus Plenodomus destruens. Biological
control of plant pathogens by Bacillus species is widely disseminated in agrosystems, but specific
biological control agents against the foot rot disease-causing fungus are not yet available. Our
previous studies showed that two Bacillus strains isolated from sweet potato roots—B. safensis
T052-76 and B. velezensis T149-19—were able to inhibit P. destruens in vitro, but data from in vivo
experiments using simultaneously the fungus and the bacteria were missing. In this study, both
strains were shown to protect the plant from the disease and to mitigate the symptoms of foot rot
disease in pot experiments. Total fungal community quantification using real-time PCR showed
a significant decrease in the number of copies of the ITS gene when the bacteria were inoculated,
compared to the control (with the fungus only). To determine the genes encoding antimicrobial
substances likely to inhibit the fungus, their genomes were sequenced and annotated. Genes coding
for mycosubtilin, bacillaene, macrolactin, bacillibactin, bacilysin, plantazolicin, plipastatin, dificidine,
fengycin and surfactin were found in B. velezensis T149-19, while those coding for bacylisin, lichenysin,
bacillibactin, fengycin and surfactin were found in B. safensis T052-76. Altogether, the data presented
here contribute to advancing the knowledge for the use of these Bacillus strains as biocontrol products
in sweet potato. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brasil. | pt_BR |
Affilliation | Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brasil. | pt_BR |
Affilliation | Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brasil. | pt_BR |
Affilliation | Universidade Federal do Sergipe. Departamento de Engenharia Agronômica do Campus de São Cristóvão. Sergipe, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Bacillus velezensis | pt_BR |
Subject | Bacillus safensis | pt_BR |
Subject | Plenodomus destruens | pt_BR |
Subject | Foot rot disease | pt_BR |
Subject | Biocontrol | pt_BR |
Subject | Sweet potato | pt_BR |