| Author | Farias, Felipe Miceli de | |
| Author | Teixeira, Lúcia Martins | |
| Author | Silva, Deyse Christina Vallim da | |
| Author | Bastos, Maria do Carmo de Freire | |
| Author | Miguel, Marco Antônio Lemos | |
| Author | Bonelli, Raquel Regina | |
| Access date | 2021-11-17T13:27:39Z | |
| Available date | 2021-11-17T13:27:39Z | |
| Document date | 2021 | |
| Citation | FARIAS, Felipe Miceli de et al. Characterization of enterococcus faecium E86 bacteriocins and their inhibition properties against listeria monocytogenes and vancomycin-resistant enterococcus. Brazilian Journal of Microbiology, v. 52, p. 1513-1522, 26 Apr. 2021. | |
| ISSN | 1517-8382 | |
| URI | https://www.arca.fiocruz.br/handle/icict/49855 | |
| Description | Produção científica do Laboratório de Zoonoses Bacterianas. | pt_BR |
| Language | eng | en_US |
| Publisher | Sociedade Brasileira de Microbiologia | |
| Rights | restricted access | |
| Title | Characterization of enterococcus faecium E86 bacteriocins and their inhibition properties against listeria monocytogenes and vancomycin-resistant enterococcus | en_US |
| Type | Article | |
| DOI | 10.1007/s42770-021-00494-3 | |
| Abstract | In the present scenario of a major demand for new compounds with antimicrobial activity, bacteriocin and bacteriocin-like inhibitory substances (BLIS) are promising tools against deteriorating and pathogenic microorganisms, thus having potential applications in both the food industry and infectious disease control. In the present report, we describe the genetic and phenotypic characteristics of BLIS produced by Enterococcus faecium E86, a strain previously isolated and sequenced by our group, focusing on the structural genes of two bacteriocins identified: enterocin TW21 and enterocin P. Transcription of all four genes associated with the biosynthesis and immunity of enterocin P and enterocin TW21 were confirmed by RT-PCR. However, Sanger sequencing confirmed a truncation of the structural gene of enterocin TW21 due to one base pair deletion (A/T). Thus, although E. faecium E86 was shown to carry two bacteriocinogenic gene clusters, only one cluster encodes a functional bacteriocin, enterocin P. Enterocin P was able to inhibit different strains of Listeria monocytogenes and vancomycin-resistant enterococci (both Enterococcus faecalis and Enterococcus faecium), showing intense bacteriolytic activity, in most cases. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil. | |
| Subject | Bacteriocin | en_US |
| Subject | Enterocin P | en_US |
| Subject | Inhibitory activity | en_US |
| Subject | Listeria monocytogenes | en_US |
| Subject | VRE | en_US |
| e-ISSN | 1678-4405 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
| xmlui.metadata.dc.subject.ods | 12 Consumo e produção responsáveis | |
