Author | Oliveira, Viviane Klassen de | |
Author | Camargo, Bruno Dias | |
Author | Alexandrino, Fabiana | |
Author | Morello, Luis Gustavo | |
Author | Marchini, Fabrício Klerynton | |
Author | Aoki, Mateus Nóbrega | |
Author | Blanes, Lucas | |
Access date | 2021-11-24T17:31:57Z | |
Available date | 2021-11-24T17:31:57Z | |
Document date | 2021 | |
Citation | OLIVEIRA, Viviane Klassen de et al. A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters. Biomedical Microdevices, v.23, n.24, p. 1–8, 2021. | pt_BR |
ISSN | 1572-8781 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50024 | |
Language | por | pt_BR |
Publisher | Springer | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | PCR | pt_BR |
Title | A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s10544-021-00563-2 | |
Abstract | This article describes the fabrication of a low-cost Polymerase Chain Reaction (PCR) instrument to detect diseases. In order to reduce the instrument price and simplify construction we developed an alternative fabrication process, transforming conventional printed circuit boards (PCB) in heating elements, avoiding the use of aluminum heating/cooling blocks and Peltier devices. To cool down the reaction a simple computer fan was used. The vial holder was fabricated using two double side PCB boards assembled in a sandwich-like confguration. The bottom PCB has a resistance of 0.9 Ω used to heat the reaction mix, while the top layer has a resistance of 1.1 Ω to heat the vial body, preventing vapor condensation. The top board was maintained
at~110±1 °C during all cycles. The fnal device was able to heat and cool down the reaction at rates of~2.0 °C/s, a rate comparable to commercial thermocyclers. An SMD NTC thermistor was used as temperature sensors, and a PID (proportional–integral–derivative) control algorithm was implemented to acquire and precisely control the temperature. We also discuss how the instrument is calibrated. The device was tested successfully for the amplifcation of T. pallidum (Syphilis) bacterial DNA and Zika virus RNA samples, showing similar performance to a commercial PCR instrument. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Subject | Polymerase Chain Reaction | pt_BR |
Subject | Reagent Kits, Diagnostic | pt_BR |
Subject in Spanish | Reacción en Cadena de la Polimerasa | pt_BR |
Subject in Spanish | Juego de Reactivos para Diagnóstico | pt_BR |
Subject in French | Réaction de polymérisation en chaîne | pt_BR |
Subject in French | Trousses de réactifs pour diagnostic | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Kit de Reagentes para Diagnóstico | pt_BR |